PMID- 101549
OWN - NLM
STAT- MEDLINE
DCOM- 19790124
LR  - 20061115
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 253
IP  - 23
DP  - 1978 Dec 10
TI  - Isolation and characterization of a high molecular weight glycoprotein from human
      blood platelets.
PG  - 8609-16
AB  - A high molecular weight glycoprotein consisting of three disulfide-linked 142,000
      molecular weight chains has been isolated from human blood platelets. The
      glycoprotein, designated thrombospondin, is released by platelets in response to 
      thrombin treatment and is proteolyzed when left in the presence of platelets
      after liberation. It is relatively insensitive to degradation by thrombin.
      Thrombospondin is a filamentous protein of dimensions approximately 7 X 70 nm and
      contains 1.9% neutral sugars, 1.4% amino sugars, 0.7% sialic acid, and no
      hexuronic acid. Amino acid analysis reveals that the level of cysteine is
      approximately 260 residues per molecule. Thrombospondin binds to immobilized
      heparin but is released by 0.45 M sodium chloride. A single band is obtained by
      isoelectric focusing, indicating a pI of 4.7 as well as a relatively high degree 
      of purity. Degradation of the intact molecule with trypsin yields a stable core
      particle of molecular weight 210,000 comprised of three 70,000 chains.
FAU - Lawler, J W
AU  - Lawler JW
FAU - Slayter, H S
AU  - Slayter HS
FAU - Coligan, J E
AU  - Coligan JE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amino Acids)
RN  - 0 (Glycoproteins)
SB  - IM
MH  - Alkylation
MH  - Amino Acids/analysis
MH  - Blood Platelets/*analysis
MH  - Glycoproteins/*blood/isolation & purification
MH  - Humans
MH  - Immunodiffusion
MH  - Microscopy, Electron
MH  - Molecular Weight
MH  - Oxidation-Reduction
MH  - Protein Conformation
EDAT- 1978/12/10 00:00
MHDA- 1978/12/10 00:01
CRDT- 1978/12/10 00:00
PHST- 1978/12/10 00:00 [pubmed]
PHST- 1978/12/10 00:01 [medline]
PHST- 1978/12/10 00:00 [entrez]
PST - ppublish
SO  - J Biol Chem. 1978 Dec 10;253(23):8609-16.