PMID- 10103055
OWN - NLM
STAT- MEDLINE
DCOM- 19990519
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 261
IP  - 1
DP  - 1999 Apr
TI  - Acn9 is a novel protein of gluconeogenesis that is located in the mitochondrial
      intermembrane space.
PG  - 236-43
AB  - Previous studies have indicated that the Acn9 protein is involved in
      gluconeogenesis. Yeast mutants defective in the ACN9 gene display phenotypes
      identical with mutants defective in metabolic enzymes required for carbon
      assimilation. These phenotypes include the inability to utilize acetate as a
      carbon and energy source, elevated levels of enzymes of the glyoxylate cycle,
      gluconeogenesis and acetyl-CoA mobilization, and a deficiency in de novo
      synthesis of glucose from ethanol. The ACN9 gene was isolated by functional
      complementation of the acetate growth defect of an acn9 mutant. The open reading 
      frame corresponds to YDR511w, and encodes a protein of unknown function. Homologs
      have been identified in human, mouse, and nematode databases. Two mutant alleles 
      were sequenced. The mutations altered amino acid residues that are conserved
      among members of the new gene family. ACN9 gene expression was slightly repressed
      by glucose, and the level of the transcript was approximately 100-fold lower than
      that of glyoxylate or tricarboxylic acid cycle enzymes. A functional
      epitope-tagged form of Acn9 was expressed to study expression and the subcellular
      localization of the protein. The tagged protein was localized to the
      mitochondrial intermembrane space.
FAU - Dennis, R A
AU  - Dennis RA
AD  - Department of Biochemistry and Molecular Biology, University of Arkansas for
      Medical Sciences, Little Rock, AK, USA.
FAU - McCammon, M T
AU  - McCammon MT
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA Primers)
RN  - 0 (Fungal Proteins)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Messenger)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carbon/metabolism
MH  - Conserved Sequence
MH  - DNA Primers/genetics
MH  - Fungal Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Fungal
MH  - Genes, Fungal
MH  - Genetic Complementation Test
MH  - *Gluconeogenesis
MH  - Humans
MH  - Mice
MH  - Mitochondria/metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Open Reading Frames
MH  - Phenotype
MH  - RNA, Fungal/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00267.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Apr;261(1):236-43. doi: 10.1046/j.1432-1327.1999.00267.x.