PMID- 10103005
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 260
IP  - 3
DP  - 1999 Mar
TI  - Molecular cloning, structural characterization and chromosomal localization of
      human lipoyltransferase gene.
PG  - 761-7
AB  - Lipoyltransferase catalyzes the transfer of the lipoyl group from lipoyl-AMP to
      the lysine residue of the lipoate-dependent enzymes. We isolated human
      lipoyltransferase cDNA and genomic DNA. The cDNA insert contained a 1119-base
      pair open reading frame encoding a precursor peptide of 373 amino acids.
      Predicted amino acid sequence of the protein shares 88 and 31% identity with
      bovine lipoyltransferase and Escherichia coli lipoate-protein ligase A,
      respectively. Northern blot analyses of poly(A)+ RNA indicated a major species of
      about 1.5 kb. mRNA levels of lipoyltransferase were highest in skeletal muscle
      and heart, showing good correlation with those of dihydrolipoamide
      acyltransferase subunits of pyruvate, 2-oxoglutarate and branched-chain 2-oxo
      acid dehydrogenase complexes and H-protein of the glycine cleavage system which
      accept lipoic acid as a prosthetic group. The human lipoyltransferase gene is a
      single copy gene composed of four exons and three introns spanning approximately 
      8 kb of genomic DNA. Some alternatively spliced mRNA species were found by
      5'-RACE analysis, and the most abundant species lacks the third exon. The human
      lipoyltransferase gene was localized to chromosome band 2q11.2 by fluorescence in
      situ hybridization.
FAU - Fujiwara, K
AU  - Fujiwara K
AD  - Institute for Enzyme Research, University of Tokushima, Japan.
FAU - Suzuki, M
AU  - Suzuki M
FAU - Okumachi, Y
AU  - Okumachi Y
FAU - Okamura-Ikeda, K
AU  - Okamura-Ikeda K
FAU - Fujiwara, T
AU  - Fujiwara T
FAU - Takahashi, E
AU  - Takahashi E
FAU - Motokawa, Y
AU  - Motokawa Y
LA  - eng
SI  - GENBANK/AB017566
SI  - GENBANK/AB017567
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.3.- (Acyltransferases)
SB  - IM
MH  - Acyltransferases/*genetics
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 2
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis
MH  - Genome, Human
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Tissue Distribution
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00204.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Mar;260(3):761-7. doi: 10.1046/j.1432-1327.1999.00204.x.