PMID- 10102990
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 260
IP  - 3
DP  - 1999 Mar
TI  - A novel form of human neuropsin, a brain-related serine protease, is generated by
      alternative splicing and is expressed preferentially in human adult brain.
PG  - 627-34
AB  - We have cloned cDNAs encoding two isoforms of a human novel serine protease. They
      encoded sequences of 260 and 305 amino acids, and both showed significant
      homology to mouse neuropsin. Mouse neuropsin has been reported to be involved in 
      hippocampal plasticity, therefore we designated the proteins as type 1 and type 2
      neuropsin, respectively. The amino acid sequences of the two types of human
      neuropsin were identical, except that type 2 carried an insert of 45 amino acids 
      at the C-terminus of the leader sequence. The essential three amino acids in the 
      active site triad, His, Asp, and Ser, and the single putative N-glycosylation
      site were conserved in human and mouse neuropsin. Sequence analysis of the 946 bp
      genomic DNA spanning the region encoding the insertion sequence revealed that two
      isoforms were generated in human brain by alternative splicing. However, the
      mouse genomic sequence did not conserve the 3' acceptor consensus sequence at the
      corresponding position, suggesting that type 2 neuropsin was a species-specific
      splice variant. When the open reading frames of human neuropsin were expressed in
      insect cells, both types of neuropsin were detected in the conditioned media by
      western blot analysis using anti-human neuropsin serum. Northern blot
      hybridization and reverse transcription-polymerase chain reaction showed
      predominant expression of type 1 neuropsin in pancreas. Type 2 neuropsin was
      preferentially expressed in human adult brain and hippocampus, although both
      types were expressed in fetal brain and placenta in comparable amounts. Dot blot 
      hybridization showed that neuropsin was expressed in various regions of adult
      brain, including the hippocampus and cerebral cortex, and also in various fetal
      tissues. These results suggest that human type 2 neuropsin may be important to
      the adult brain plasticity, although both types may be necessary for the
      development of the nervous system.
FAU - Mitsui, S
AU  - Mitsui S
AD  - Department of Cell Biology, Institute for Neurological Diseases and Geriatrics,
      Kawaramachi Hirokaji, Japan.
FAU - Tsuruoka, N
AU  - Tsuruoka N
FAU - Yamashiro, K
AU  - Yamashiro K
FAU - Nakazato, H
AU  - Nakazato H
FAU - Yamaguchi, N
AU  - Yamaguchi N
LA  - eng
SI  - GENBANK/AB008390
SI  - GENBANK/AB008927
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.21.- (KLK8 protein, human)
RN  - EC 3.4.21.- (Kallikreins)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Adult
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*enzymology
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis
MH  - Humans
MH  - Insecta
MH  - *Kallikreins
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Serine Endopeptidases/biosynthesis/*genetics/metabolism
MH  - Species Specificity
MH  - Tissue Distribution
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00213.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Mar;260(3):627-34. doi: 10.1046/j.1432-1327.1999.00213.x.