PMID- 10102820
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 284
IP  - 5411
DP  - 1999 Apr 2
TI  - Dynamic control of CaMKII translocation and localization in hippocampal neurons
      by NMDA receptor stimulation.
PG  - 162-6
AB  - Calcium-calmodulin-dependent protein kinase II (CaMKII) is thought to increase
      synaptic strength by phosphorylating postsynaptic density (PSD) ion channels and 
      signaling proteins. It is shown that N-methyl-D-aspartate (NMDA) receptor
      stimulation reversibly translocates green fluorescent protein-tagged CaMKII from 
      an F-actin-bound to a PSD-bound state. The translocation time was controlled by
      the ratio of expressed beta-CaMKII to alpha-CaMKII isoforms. Although F-actin
      dissociation into the cytosol required autophosphorylation of or
      calcium-calmodulin binding to beta-CaMKII, PSD translocation required binding of 
      calcium-calmodulin to either the alpha- or beta-CaMKII subunits.
      Autophosphorylation of CaMKII indirectly prolongs its PSD localization by
      increasing the calmodulin-binding affinity.
FAU - Shen, K
AU  - Shen K
AD  - Department of Cell Biology and Department of Pharmacology and Cancer Biology, Box
      3709, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Meyer, T
AU  - Meyer T
LA  - eng
GR  - GM-48113/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Actins)
RN  - 0 (Isoenzymes)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (postsynaptic density proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Calcium/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cells, Cultured
MH  - Cytosol/metabolism
MH  - Dendrites/*enzymology
MH  - Electric Stimulation
MH  - Glutamic Acid/pharmacology
MH  - Green Fluorescent Proteins
MH  - Hippocampus/cytology/*enzymology
MH  - Isoenzymes/metabolism
MH  - Luminescent Proteins
MH  - Microscopy, Fluorescence
MH  - Nerve Tissue Proteins/analysis
MH  - Neurons/*enzymology
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Synapses/*enzymology
MH  - Tumor Cells, Cultured
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1126/science.284.5411.162 [doi]
PST - ppublish
SO  - Science. 1999 Apr 2;284(5411):162-6. doi: 10.1126/science.284.5411.162.