PMID- 10102268
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 6
DP  - 1999 Mar 19
TI  - Slit proteins bind Robo receptors and have an evolutionarily conserved role in
      repulsive axon guidance.
PG  - 795-806
AB  - Extending axons in the developing nervous system are guided in part by repulsive 
      cues. Genetic analysis in Drosophila, reported in a companion to this paper,
      identifies the Slit protein as a candidate ligand for the repulsive guidance
      receptor Roundabout (Robo). Here we describe the characterization of three
      mammalian Slit homologs and show that the Drosophila Slit protein and at least
      one of the mammalian Slit proteins, Slit2, are proteolytically processed and show
      specific, high-affinity binding to Robo proteins. Furthermore, recombinant Slit2 
      can repel embryonic spinal motor axons in cell culture. These results support the
      hypothesis that Slit proteins have an evolutionarily conserved role in axon
      guidance as repulsive ligands for Robo receptors.
FAU - Brose, K
AU  - Brose K
AD  - Department of Anatomy, Howard Hughes Medical Institute, University of California,
      San Francisco 94143-0452, USA.
FAU - Bland, K S
AU  - Bland KS
FAU - Wang, K H
AU  - Wang KH
FAU - Arnott, D
AU  - Arnott D
FAU - Henzel, W
AU  - Henzel W
FAU - Goodman, C S
AU  - Goodman CS
FAU - Tessier-Lavigne, M
AU  - Tessier-Lavigne M
FAU - Kidd, T
AU  - Kidd T
LA  - eng
SI  - GENBANK/AF133270
SI  - GENBANK/AF133730
SI  - GENBANK/AF182037
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Complementary)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Robo2 protein, rat)
RN  - 0 (roundabout protein)
RN  - 0 (sli protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Axons/*physiology
MH  - Base Sequence
MH  - COS Cells
MH  - Cell Line, Transformed
MH  - *Conserved Sequence
MH  - DNA, Complementary
MH  - Drosophila
MH  - *Drosophila Proteins
MH  - *Evolution, Molecular
MH  - Humans
MH  - Insect Proteins/genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Motor Neurons/physiology
MH  - Nerve Tissue Proteins/genetics/metabolism/*physiology
MH  - Protein Processing, Post-Translational
MH  - Rats
MH  - Receptors, Immunologic/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Spinal Cord
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - S0092-8674(00)80590-5 [pii]
AID - 10.1016/s0092-8674(00)80590-5 [doi]
PST - ppublish
SO  - Cell. 1999 Mar 19;96(6):795-806. doi: 10.1016/s0092-8674(00)80590-5.