PMID- 10102266
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 6
DP  - 1999 Mar 19
TI  - Biochemical purification of a mammalian slit protein as a positive regulator of
      sensory axon elongation and branching.
PG  - 771-84
AB  - Many neurons in both vertebrates and invertebrates innervate multiple targets by 
      sprouting secondary axon collaterals (or branches) from a primary axon shaft. To 
      begin to identify molecular regulators of axon branch initiation or extension, we
      studied the growth of single sensory axons in an in vitro collagen assay system
      and identified an activity in extracts of embryonic spinal cord and of postnatal 
      and adult brain that promotes the elongation and formation of extensive branches 
      by these axons. Biochemical purification of the activity from calf brain extracts
      led to the identification of an amino-terminal fragment of Slit2 as the main
      active component and to the discovery of a distinct activity that potentiates its
      effects. These results indicate that Slit proteins may function as positive
      regulators of axon collateral formation during the establishment or remodeling of
      neural circuits.
FAU - Wang, K H
AU  - Wang KH
AD  - Department of Anatomy, Howard Hughes Medical Institute, University of California,
      San Francisco 94143-0452, USA.
FAU - Brose, K
AU  - Brose K
FAU - Arnott, D
AU  - Arnott D
FAU - Kidd, T
AU  - Kidd T
FAU - Goodman, C S
AU  - Goodman CS
FAU - Henzel, W
AU  - Henzel W
FAU - Tessier-Lavigne, M
AU  - Tessier-Lavigne M
LA  - eng
SI  - GENBANK/AF133270
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (roundabout protein)
RN  - R6FXH13RRC (Slit homolog 2 protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Axons/*physiology
MH  - Brain/embryology
MH  - COS Cells
MH  - Cattle
MH  - Cell Division
MH  - Gene Expression
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*physiology
MH  - Rats
MH  - Receptors, Immunologic/genetics
MH  - Spinal Cord/embryology
MH  - Time Factors
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - S0092-8674(00)80588-7 [pii]
AID - 10.1016/s0092-8674(00)80588-7 [doi]
PST - ppublish
SO  - Cell. 1999 Mar 19;96(6):771-84. doi: 10.1016/s0092-8674(00)80588-7.