PMID- 10101301
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 4
DP  - 1999 Apr
TI  - Characterization, cDNA cloning, and functional expression of mouse ileal
      sodium-dependent bile acid transporter.
PG  - 846-51
AB  - Mouse ileal sodium dependent bile acid transporter (ISBT) was characterized using
      isolated enterocytes. Only enterocytes from the most distal portion showed
      Na+-dependent [3H]taurocholate uptake. Northern blot analysis using a probe
      against mouse ISBT revealed the expression of mouse ISBT mRNA to be restricted to
      the distal ileum. The Km and Vmax for Na+-dependent [3H]taurocholate transport
      into isolated ileocytes were calculated as 27 microM and 360 pmol/mg protein/min,
      respectively. Uptake of [3H]taurocholate was inhibited by N-ethylmaleimide. We
      have cloned ISBT cDNA from mouse ileum. The cDNA included the entire open reading
      frame coding 348 amino acid protein with seven hydrophobic segments and two
      N-glycosylation sites. COS-7 cells transfected with the expression vector
      containing this cDNA expressed Na+-dependent [3H]taurocholate uptake activity
      with a Km of 34 microM.
FAU - Saeki, T
AU  - Saeki T
AD  - Laboratory of Molecular Nutrition, Department of Biological Resource Chemistry,
      Kyoto Prefectural University, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan.
      tsaeki@dns.kpu.ac.jp
FAU - Matoba, K
AU  - Matoba K
FAU - Furukawa, H
AU  - Furukawa H
FAU - Kirifuji, K
AU  - Kirifuji K
FAU - Kanamoto, R
AU  - Kanamoto R
FAU - Iwami, K
AU  - Iwami K
LA  - eng
SI  - GENBANK/AB002693
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Organic Anion Transporters, Sodium-Dependent)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Symporters)
RN  - 145420-23-1 (sodium-bile acid cotransporter)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Bile Acids and Salts/*metabolism
MH  - Biological Transport, Active
MH  - COS Cells
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/*genetics
MH  - Gene Expression
MH  - Humans
MH  - Ileum/*metabolism
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Molecular Sequence Data
MH  - *Organic Anion Transporters, Sodium-Dependent
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Sodium/*metabolism
MH  - *Symporters
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022358 [doi]
PST - ppublish
SO  - J Biochem. 1999 Apr;125(4):846-51. doi: 10.1093/oxfordjournals.jbchem.a022358.