PMID- 10101292
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20191210
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 4
DP  - 1999 Apr
TI  - Dynamin II is involved in endocytosis but not in the formation of transport
      vesicles from the trans-Golgi network.
PG  - 780-9
AB  - Dynamins are a family of approximately 100-kDa GTPases that are thought to play a
      pivotal role in the formation of endocytic coated vesicles. There are three
      dynamin genes in mammals: dynamin I is neuron-specific, dynamin II shows
      ubiquitous expression, and dynamin III is expressed in testis, brain, and lung.
      However, most studies on the functions of dynamins to date have been restricted
      to dynamin I. In the present study, we show that, like dynamin I, dynamin II is
      involved in receptor-mediated endocytosis. While this study was in progress,
      Jones et al. [Jones, S.M., Howell, K.E., Henley, J.R., Cao, H., and McNiven, M.A.
      (1998) Science 279, 573-577] reported that dynamin II is localized in the
      trans-Golgi network (TGN) and involved in the formation of constitutive transport
      vesicles and clathrin-coated vesicles from this compartment. However,
      immunofluorescence analyses and experiments using cells transfected with
      dominant-negative dynamin II failed to show any evidence for localization of
      dynamin II in the TGN or for its involvement in vesicle formation from this
      compartment. Our data thus indicate that dynamin II is involved in endocytosis
      but not in the formation of transport vesicles from the TGN.
FAU - Kasai, K
AU  - Kasai K
AD  - Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Science City,
      Ibaraki, 305-8572, Japan.
FAU - Shin, H W
AU  - Shin HW
FAU - Shinotsuka, C
AU  - Shinotsuka C
FAU - Murakami, K
AU  - Murakami K
FAU - Nakayama, K
AU  - Nakayama K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Recombinant Proteins)
RN  - EC 3.5.1.50 (Dynamin I)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.5 (Dynamin III)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Dynamin I
MH  - Dynamin III
MH  - Dynamins
MH  - Endocytosis/*physiology
MH  - Fluorescent Antibody Technique, Indirect
MH  - GTP Phosphohydrolases/genetics/*metabolism
MH  - Golgi Apparatus/*enzymology
MH  - HeLa Cells
MH  - Humans
MH  - Male
MH  - Point Mutation
MH  - Recombinant Proteins/genetics/metabolism
MH  - Subcellular Fractions/enzymology
MH  - Transfection
MH  - Vero Cells
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022349 [doi]
PST - ppublish
SO  - J Biochem. 1999 Apr;125(4):780-9. doi: 10.1093/oxfordjournals.jbchem.a022349.