PMID- 10101285
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 4
DP  - 1999 Apr
TI  - Characterization of the novel mitochondrial protein import component, Tom34, in
      mammalian cells.
PG  - 721-7
AB  - Tom34 is a newly-found component of the mitochondrial protein import machinery in
      mammalian cells with no apparent counterpart in fungi. RNA blot and immunoblot
      analyses showed that the expression of Tom34 varies among tissues and differs
      from that of the core translocase component Tom20. In contrast to a previous
      report [Nuttal, S.D. et al. (1997) DNA Cell Biol. 16, 1067-1074], the present
      study using a newly-prepared anti-Tom34 antibody with a high titer showed that
      Tom34 is present largely in the cytosolic fraction and partly in the
      mitochondrial and membrane fractions after fractionation of tissues and cells,
      and that the membrane-associated form is largely extractable with 0.1 M sodium
      carbonate. The in vitro import of preproteins into isolated rat mitochondria was 
      strongly inhibited by DeltahTom34 which lacks the NH2-terminal hydrophobic region
      of human Tom34 (hTom34). Import was also strongly inhibited by anti-hTom34. In
      pulse-chase experiments using COS-7 cells, pre-ornithine transcarbamylase (pOTC) 
      was rapidly processed to the mature form. Coexpression of hTom34 resulted in a
      stimulation of pOTC processing, whereas the coexpression of hTom34 antisense RNA 
      caused inhibition. The results confirm that Tom34 plays a role in mitochondrial
      protein import in mammals, and suggest it to be an ancillary component of the
      translocation machinery in mammalian cells.
FAU - Chewawiwat, N
AU  - Chewawiwat N
AD  - Department of Molecular Genetics, Kumamoto University School of Medicine,
      Kumamoto, 860-0811, Japan.
FAU - Yano, M
AU  - Yano M
FAU - Terada, K
AU  - Terada K
FAU - Hoogenraad, N J
AU  - Hoogenraad NJ
FAU - Mori, M
AU  - Mori M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Membrane Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mitochondrial Membrane Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TOMM20 protein, human)
RN  - 0 (TOMM34 protein, human)
RN  - 0 (Tomm20 protein, rat)
RN  - EC 2.1.3.3 (Ornithine Carbamoyltransferase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cytosol/metabolism
MH  - DNA Primers/genetics
MH  - Enzyme Precursors/metabolism
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - *Membrane Transport Proteins
MH  - Membranes/metabolism
MH  - Mitochondria/*metabolism
MH  - *Mitochondrial Membrane Transport Proteins
MH  - Ornithine Carbamoyltransferase/metabolism
MH  - Pregnancy
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - *Receptors, Cell Surface
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Swine
MH  - Tissue Distribution
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022342 [doi]
PST - ppublish
SO  - J Biochem. 1999 Apr;125(4):721-7. doi: 10.1093/oxfordjournals.jbchem.a022342.