PMID- 10101268
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1437
IP  - 3
DP  - 1999 Mar 25
TI  - Functional expression and characterisation of a new human phosphatidylinositol
      4-kinase PI4K230.
PG  - 341-56
AB  - By constructing DNA probes we have identified and cloned a human PtdIns 4-kinase,
      PI4K230, corresponding to a mRNA of 7.0 kb. The cDNA encodes a protein of 2044
      amino acids. The C-terminal part of ca. 260 amino acids represents the catalytic 
      domain which is highly conserved in all recently cloned PtdIns 4-kinases.
      N-terminal motifs indicate multiple heterologous protein interactions. Human
      PtdIns 4-kinase PI4K230 expressed in vitro exhibits a specific activity of 58
      micromol mg-1min-1. The enzyme expressed in Sf9 cells is essentially not
      inhibited by adenosine, it shows a high Km for ATP of about 300 microM and it is 
      half-maximally inactivated by approximately 200 nM wortmannin. These data
      classify this enzyme as type 3 PtdIns 4-kinase. Antibodies raised against the
      N-terminal part moderately activate and those raised against the C-terminal
      catalytic domain inhibit the enzymatic activity. The coexistence of two different
      type 3 PtdIns 4-kinases, PI4K92 and PI4K230, in several human tissues, including 
      brain, suggests that these enzymes are involved in distinct basic cellular
      functions.
FAU - Gehrmann, T
AU  - Gehrmann T
AD  - Ruhr-Universitat Bochum, Institut fur Physiologische Chemie, Abteilung fur
      Biochemie Supramolekularer Systeme, D-44780, Bochum, Germany.
FAU - Gulkan, H
AU  - Gulkan H
FAU - Suer, S
AU  - Suer S
FAU - Herberg, F W
AU  - Herberg FW
FAU - Balla, A
AU  - Balla A
FAU - Vereb, G
AU  - Vereb G
FAU - Mayr, G W
AU  - Mayr GW
FAU - Heilmeyer, L M Jr
AU  - Heilmeyer LM Jr
LA  - eng
SI  - GENBANK/UNKNOWN
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Isoenzymes)
RN  - EC 2.7.1.67 (1-Phosphatidylinositol 4-Kinase)
SB  - IM
MH  - 1-Phosphatidylinositol 4-Kinase/biosynthesis/chemistry/*genetics
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Humans
MH  - Isoenzymes/biosynthesis/chemistry/genetics
MH  - Molecular Sequence Data
EDAT- 1999/04/02 03:02
MHDA- 2001/03/28 10:01
CRDT- 1999/04/02 03:02
PHST- 1999/04/02 03:02 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/04/02 03:02 [entrez]
AID - S1388-1981(99)00029-3 [pii]
AID - 10.1016/s1388-1981(99)00029-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Mar 25;1437(3):341-56. doi:
      10.1016/s1388-1981(99)00029-3.