PMID- 10101267
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1437
IP  - 3
DP  - 1999 Mar 25
TI  - Identification of the GGPS1 genes encoding geranylgeranyl diphosphate synthases
      from mouse and human.
PG  - 333-40
AB  - E,E,E-Geranylgeranyl diphosphate (GGPP) is an important precursor of carotenoids 
      and geranylgeranylated proteins such as small G proteins. In this study, we have 
      identified mouse and human GGPP synthase genes. Sequence analysis showed that
      mouse and human GGPP synthases share a high level of amino acid identity (94%)
      with each other, and share a high level of similarity (45-50%) with GGPP
      synthases of lower eukaryotes, but only weak similarity (22-31%) to plant and
      prokaryotic GGPP synthases. Both of the newly identified GGPP synthase genes from
      mouse and human were expressed in Escherichia coli, and their gene products
      displayed GGPP synthase activity when isopentenyl diphosphate and farnesyl
      diphosphate were used as substrates. The GGPP synthase activity of these genes
      was also confirmed by demonstrating carotenoid synthesis after co-transformation 
      of E. coli with a plasmid expressing the crt genes derived from Erwinia
      uredovora, and a plasmid expressing either the mouse or human GGPS1 gene.
      Southern blot analysis suggests that the human GGPS1 gene is a single copy gene.
FAU - Kainou, T
AU  - Kainou T
AD  - Department of Applied Bioscience and Biotechnology, Faculty of Life and
      Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue 690-8504,
      Japan.
FAU - Kawamura, K
AU  - Kawamura K
FAU - Tanaka, K
AU  - Tanaka K
FAU - Matsuda, H
AU  - Matsuda H
FAU - Kawamukai, M
AU  - Kawamukai M
LA  - eng
SI  - GENBANK/AB016043
SI  - GENBANK/AB016044
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Ggps1 protein, mouse)
RN  - 0 (Multienzyme Complexes)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.1 (Dimethylallyltranstransferase)
RN  - EC 2.5.1.10 (Geranyltranstransferase)
RN  - EC 2.5.1.29 (Farnesyltranstransferase)
RN  - EC 2.5.1.29 (GGPS1 protein, human)
SB  - IM
MH  - Alkyl and Aryl Transferases/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Southern
MH  - Dimethylallyltranstransferase
MH  - Escherichia coli/enzymology/*genetics
MH  - Farnesyltranstransferase
MH  - Geranyltranstransferase
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes
MH  - Phylogeny
MH  - Plasmids
MH  - Sequence Alignment
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - S1388-1981(99)00028-1 [pii]
AID - 10.1016/s1388-1981(99)00028-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Mar 25;1437(3):333-40. doi:
      10.1016/s1388-1981(99)00028-1.