PMID- 10101262
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1437
IP  - 3
DP  - 1999 Mar 25
TI  - Transacylase formation of bis(monoacylglycerol)phosphate.
PG  - 277-86
AB  - Recent work within our laboratory has focused on the enzymes we hypothesize are
      involved in the biosynthesis of bis(monoacylglycerol)phosphate from
      phosphatidylglycerol. Here we describe a transacylase, active at acidic pH
      values, isolated from a macrophage-like cell line, RAW 264.7. This enzyme
      acylates the head group glycerol of sn-3:sn-1' lysophosphatidylglycerol to form
      sn-3:sn-1' bis(monoacylglycerol)phosphate. Here we demonstrate that this enzyme
      uses two lysophosphatidylglycerol molecules, one as an acyl donor and another as 
      an acyl acceptor, and that the acyl contributions from all other lipids tested
      are comparatively minor. This enzyme prefers saturated acyl chains to
      monounsaturates, 16 and 18 carbon fatty acids over 14 carbon fatty acids, and
      saturated acyl chains at the sn-1 position to monounsaturated acyl chains on the 
      sn-2 carbon of lysophosphatidylglycerol. We present data which show the
      transacylase activity depends on the presence of a lipid-water interface and the 
      lipid polymorphic state.
FAU - Heravi, J
AU  - Heravi J
AD  - Department of Biochemistry, Wake Forest University School of Medicine, Medical
      Center Boulevard, Winston-Salem, NC 27157-1019, USA.
FAU - Waite, M
AU  - Waite M
LA  - eng
GR  - CA-12197/CA/NCI NIH HHS/United States
GR  - HL-50395/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Lysophospholipids)
RN  - 0 (Monoglycerides)
RN  - 0 (Phosphatidylglycerols)
RN  - 0 (bis(monoacylglyceryl)phosphate)
RN  - 0 (lysophosphatidylglycerol)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.22 (2-acylglycerol O-acyltransferase)
SB  - IM
MH  - Acyltransferases/isolation & purification/*metabolism
MH  - Animals
MH  - Lysophospholipids/*biosynthesis/metabolism
MH  - Macrophages/enzymology
MH  - Mice
MH  - Monoglycerides
MH  - Phosphatidylglycerols/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
AID - S1388-1981(99)00021-9 [pii]
AID - 10.1016/s1388-1981(99)00021-9 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Mar 25;1437(3):277-86. doi:
      10.1016/s1388-1981(99)00021-9.