PMID- 10101178
OWN - NLM
STAT- MEDLINE
DCOM- 19990512
LR  - 20181113
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 151
IP  - 4
DP  - 1999 Apr
TI  - Distribution of crossing over on mouse synaptonemal complexes using
      immunofluorescent localization of MLH1 protein.
PG  - 1569-79
AB  - We have used immunofluorescent localization to examine the distribution of MLH1
      (MutL homolog) foci on synaptonemal complexes (SCs) from juvenile male mice. MLH1
      is a mismatch repair protein necessary for meiotic recombination in mice, and
      MLH1 foci have been proposed to mark crossover sites. We present evidence that
      the number and distribution of MLH1 foci on SCs closely correspond to the number 
      and distribution of chiasmata on diplotene-metaphase I chromosomes. MLH1 foci
      were typically excluded from SC in centromeric heterochromatin. For SCs with one 
      MLH1 focus, most foci were located near the middle of long SCs, but near the
      distal end of short SCs. For SCs with two MLH1 foci, the distribution of foci was
      bimodal regardless of SC length, with most foci located near the proximal and
      distal ends. The distribution of MLH1 foci indicated interference between foci.
      We observed a consistent relative distance (percent of SC length in euchromatin) 
      between two foci on SCs of different lengths, suggesting that positive
      interference between MLH1 foci is a function of relative SC length. The extended 
      length of pachytene SCs, as compared to more condensed diplotene-metaphase I
      bivalents, makes mapping crossover events and interference distances using MLH1
      foci more accurate than using chiasmata.
FAU - Anderson, L K
AU  - Anderson LK
AD  - Department of Biology, Colorado State University, Fort Collins, Colorado 80523,
      USA. lorrie@lamar.colostate.edu
FAU - Reeves, A
AU  - Reeves A
FAU - Webb, L M
AU  - Webb LM
FAU - Ashley, T
AU  - Ashley T
LA  - eng
GR  - GM-49779/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Mlh1 protein, mouse)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - EC 3.6.1.3 (MutL Protein Homolog 1)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Base Pair Mismatch
MH  - Binding Sites
MH  - Carrier Proteins
MH  - *Crossing Over, Genetic
MH  - DNA Repair
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - MutL Protein Homolog 1
MH  - Neoplasm Proteins/*metabolism
MH  - Nuclear Proteins
MH  - Recombination, Genetic
MH  - Spermatocytes/metabolism/ultrastructure
MH  - Synaptonemal Complex/*genetics
PMC - PMC1460565
EDAT- 1999/04/02 00:00
MHDA- 1999/04/02 00:01
CRDT- 1999/04/02 00:00
PHST- 1999/04/02 00:00 [pubmed]
PHST- 1999/04/02 00:01 [medline]
PHST- 1999/04/02 00:00 [entrez]
PST - ppublish
SO  - Genetics. 1999 Apr;151(4):1569-79.