PMID- 10100856
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 446
IP  - 2-3
DP  - 1999 Mar 12
TI  - AlphaB-crystallin in the rat lens is phosphorylated at an early post-natal age.
PG  - 269-72
AB  - We determined the developmental changes in the phosphorylation state of
      alphaB-crystallin in lenses from rats at various post-natal ages by isoelectric
      focusing gel electrophoresis or sodium dodecyl sulfate-polyacrylamide gel
      electrophoresis and a subsequent Western blot analysis of extracts of lenses
      using antibodies that recognized the carboxy-terminal sequence or each of the
      three phosphorylated serine residues (Ser-19, Ser-45 and Ser-59) in
      alphaB-crystallin. Phosphorylated forms of alphaB-crystallin were barely detected
      at birth but they became detectable at 3 weeks of age and reached plateau levels 
      at 8 weeks of age. The phosphorylation of alphaB-crystallin at Ser-45 was
      observed preferentially. The active form of p44/42 MAP kinase, which is
      responsible for the phosphorylation of Ser-45 in alphaB-crystallin, also
      increased in a development-dependent manner. Thus we found that the developmental
      increase of the phosphorylation at Ser-45 of alphaB-crystallin in the rat lens
      was due to the developmental activation of p44/42 MAP kinase.
FAU - Ito, H
AU  - Ito H
AD  - Department of Biochemistry, Institute for Developmental Research, Aichi Human
      Service Center, Kasugai, Japan. itohide@inst-hsc.pref.aichi.jp
FAU - Iida, K
AU  - Iida K
FAU - Kamei, K
AU  - Kamei K
FAU - Iwamoto, I
AU  - Iwamoto I
FAU - Inaguma, Y
AU  - Inaguma Y
FAU - Kato, K
AU  - Kato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Crystallins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Crystallins/*metabolism
MH  - Enzyme Activation
MH  - Humans
MH  - Lens, Crystalline/*metabolism
MH  - Male
MH  - Phosphorylation
MH  - Protein Kinases/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 1999/04/01 00:00
MHDA- 1999/04/01 00:01
CRDT- 1999/04/01 00:00
PHST- 1999/04/01 00:00 [pubmed]
PHST- 1999/04/01 00:01 [medline]
PHST- 1999/04/01 00:00 [entrez]
AID - S0014-5793(99)00242-2 [pii]
AID - 10.1016/s0014-5793(99)00242-2 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Mar 12;446(2-3):269-72. doi: 10.1016/s0014-5793(99)00242-2.