PMID- 10100623
OWN - NLM
STAT- MEDLINE
DCOM- 19990507
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 446
IP  - 1
DP  - 1999 Mar 5
TI  - Discovery of a receptor related to the galanin receptors.
PG  - 103-7
AB  - We report the isolation of a cDNA clone named GPR54, which encodes a novel G
      protein-coupled receptor (GPCR). A PCR search of rat brain cDNA retrieved a clone
      partially encoding a GPCR. In a library screening this clone was used to isolate 
      a cDNA with an open reading frame (ORF) encoding a receptor of 396 amino acids
      long which shared significant identities in the transmembrane regions with rat
      galanin receptors GalR1 (45%), GalR3 (45%) and GalR2 (44%). Northern blot and in 
      situ hybridization analyses revealed that GPR54 is expressed in brain regions
      (pons, midbrain, thalamus, hypothalamus, hippocampus, amygdala, cortex, frontal
      cortex, and striatum) as well as peripheral regions (liver and intestine). In COS
      cell expression of GPR54 no specific binding was observed for 125I-galanin. A
      recent BLAST search with the rat GPR54 ORF nucleotide sequence recovered the
      human orthologue of GPR54 in a 3.5 Mb contig localized to chromosome 19p13.3.
FAU - Lee, D K
AU  - Lee DK
AD  - Department of Pharmacology, University of Toronto, Ont., Canada.
FAU - Nguyen, T
AU  - Nguyen T
FAU - O'Neill, G P
AU  - O'Neill GP
FAU - Cheng, R
AU  - Cheng R
FAU - Liu, Y
AU  - Liu Y
FAU - Howard, A D
AU  - Howard AD
FAU - Coulombe, N
AU  - Coulombe N
FAU - Tan, C P
AU  - Tan CP
FAU - Tang-Nguyen, A T
AU  - Tang-Nguyen AT
FAU - George, S R
AU  - George SR
FAU - O'Dowd, B F
AU  - O'Dowd BF
LA  - eng
SI  - GENBANK/AF115516
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (KISS1R protein, human)
RN  - 0 (Kiss1r protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Galanin)
RN  - 0 (Receptors, Kisspeptin-1)
RN  - 0 (Receptors, Neuropeptide)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoradiography
MH  - Brain/*metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis/genetics
MH  - GTP-Binding Proteins/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Organ Specificity
MH  - Rats
MH  - Receptors, G-Protein-Coupled
MH  - Receptors, Galanin
MH  - Receptors, Kisspeptin-1
MH  - Receptors, Neuropeptide/*genetics/metabolism
MH  - Sequence Alignment
EDAT- 1999/04/01 00:00
MHDA- 1999/04/01 00:01
CRDT- 1999/04/01 00:00
PHST- 1999/04/01 00:00 [pubmed]
PHST- 1999/04/01 00:01 [medline]
PHST- 1999/04/01 00:00 [entrez]
AID - S0014-5793(99)00009-5 [pii]
AID - 10.1016/s0014-5793(99)00009-5 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Mar 5;446(1):103-7. doi: 10.1016/s0014-5793(99)00009-5.