PMID- 10100302
OWN - NLM
STAT- MEDLINE
DCOM- 19990526
LR  - 20190818
IS  - 0724-8741 (Print)
IS  - 0724-8741 (Linking)
VI  - 16
IP  - 2
DP  - 1999 Feb
TI  - The effect of hormones on the expression of five isoforms of
      UDP-glucuronosyltransferase in primary cultures of rat hepatocytes.
PG  - 191-7
AB  - PURPOSE: To investigate the direct effects of sex hormones, growth hormone,
      thyroid hormones and dexamethasone on the regulation of
      UDP-glucuronosyltransferase (UGT). METHODS: Rat hepatocytes were cultured on
      matrigel and treated with various hormones. Northern blot analysis was carried
      out using cDNA probes to family 1 and family 2 isoforms. RESULTS: Treatment with 
      10(-5) M testosterone increased the mRNA levels of UGT 2B1 by 29% and UGT2B3 by
      32%. Incubation of growth hormone (10 mU) with hepatocytes suppressed the
      expression of UGT2B1 and UGT2B3 by 17% and 38%, respectively. T3 administration
      resulted in a time and dose-dependent effect on the expression of UGT 1 isoforms,
      with increased UGT1A6 by 70%, and decreased UGT1A1 by 38% and UGT1A5 by 35%. All 
      UGT isoforms except UGT 1A6 studied in this assay were up-regulated by
      dexamethasone, but to different degrees. The regulation of UGT1A1 and UGT2B1 by
      dexamethasone was dose and time dependent, and the induction of dexamethasone in 
      the expression of UGT1A1 and UGT2B1 was blocked by cycloheximide but not
      dichloro-1-D-ribofuranosylbenzimidazole. CONCLUSIONS: This study demonstrates
      that multiple hormones take part in the regulation of UGT mRNA expression in the 
      rat and individual genes can be differentially modulated.
FAU - Li, Y Q
AU  - Li YQ
AD  - Department of Gastroenterology, St. Vincent's Hospital, Fitzroy, Victoria,
      Australia.
FAU - Prentice, D A
AU  - Prentice DA
FAU - Howard, M L
AU  - Howard ML
FAU - Mashford, M L
AU  - Mashford ML
FAU - Desmond, P V
AU  - Desmond PV
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Hormones)
RN  - 0 (Isoenzymes)
RN  - 0 (Thyroid Hormones)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9002-72-6 (Growth Hormone)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Glucuronosyltransferase/*biosynthesis/metabolism
MH  - Gonadal Steroid Hormones/pharmacology
MH  - Growth Hormone/pharmacology
MH  - Hormones/*pharmacology
MH  - Isoenzymes/*biosynthesis
MH  - Liver/cytology/*drug effects/enzymology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thyroid Hormones/pharmacology
EDAT- 1999/04/01 00:00
MHDA- 1999/04/01 00:01
CRDT- 1999/04/01 00:00
PHST- 1999/04/01 00:00 [pubmed]
PHST- 1999/04/01 00:01 [medline]
PHST- 1999/04/01 00:00 [entrez]
AID - 10.1023/a:1018812021549 [doi]
PST - ppublish
SO  - Pharm Res. 1999 Feb;16(2):191-7. doi: 10.1023/a:1018812021549.