PMID- 10100195
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20190831
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 34
IP  - 1
DP  - 1999 Jan
TI  - Activity of phosphatidylethanolamine-N-methyltransferase in brain affected by
      Alzheimer's disease.
PG  - 41-7
AB  - To determine whether phospholipid abnormality in Alzheimer's disease is
      associated with modification of phosphatidylethanolamine-N-methyltransferase, the
      activity of the enzyme was analysed in the frontal and occipital cortex of the
      brain from patients with Alzheimer's disease and from aged-matched control. The
      optimum pH for phosphatidylethanolamine-N-methyltransferase in human brain was
      9.0. The enzyme activity was stimulated by detergent TWEEN 20 but inhibited by
      Triton X-100. Neither magnesium dependence nor chemical methylation was found. A 
      decrease in activity of phosphatidylethanolamine-N-methyltransferase was observed
      in the frontal cortex of brain affected with Alzheimer's disease. The addition of
      exogenous phosphatidylethanolamine resulted in no modification in the methylation
      rate as compared with that of endogenous PE. The addition of
      phosphatidyl-N-monomethylethanolamine and phosphatidyl-N,N-dimethylethanolamine
      resulted in significantly increased rates of methylation in brain tissues.
      However, the increased rate of phosphatidylethanolamine-N-methyltransferase
      activity stimulated by exogenous phospholipids was lower in the frontal cortex of
      brains with Alzheimer's disease when compared to the normals and there was no
      difference in the occipital cortex between Alzheimer's disease and the control.
      It is plausible that the decreased activity of
      phosphatidylethanolamine-N-methyltransferase and its low compensating ability
      could relate to the modification of phosphatidylcholine in brain tissues from
      Alzheimer's disease patients.
FAU - Guan, Z Z
AU  - Guan ZZ
AD  - Department of Pathology, Guiyang Medical College, China.
      zhizhong.guan@kfcmail.hs.sll.se
FAU - Wang, Y N
AU  - Wang YN
FAU - Xiao, K Q
AU  - Xiao KQ
FAU - Hu, P S
AU  - Hu PS
FAU - Liu, J L
AU  - Liu JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Polysorbates)
RN  - 0 (phosphatidyl-N-monomethylaminoethanol)
RN  - 0 (phosphatidyldimethylethanolamine)
RN  - 9002-93-1 (Octoxynol)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.17 (PEMT protein, human)
RN  - EC 2.1.1.17 (Phosphatidylethanolamine N-Methyltransferase)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/*enzymology
MH  - Brain/*enzymology
MH  - Enzyme Inhibitors/pharmacology
MH  - Frontal Lobe/enzymology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Methylation
MH  - Methyltransferases/antagonists & inhibitors/*metabolism
MH  - Occipital Lobe/enzymology
MH  - Octoxynol/pharmacology
MH  - Phosphatidylcholines/metabolism
MH  - Phosphatidylethanolamine N-Methyltransferase
MH  - Phosphatidylethanolamines/metabolism/pharmacology
MH  - Polysorbates/pharmacology
EDAT- 1999/04/01 00:00
MHDA- 1999/04/01 00:01
CRDT- 1999/04/01 00:00
PHST- 1999/04/01 00:00 [pubmed]
PHST- 1999/04/01 00:01 [medline]
PHST- 1999/04/01 00:00 [entrez]
AID - S0197-0186(98)00068-0 [pii]
AID - 10.1016/s0197-0186(98)00068-0 [doi]
PST - ppublish
SO  - Neurochem Int. 1999 Jan;34(1):41-7. doi: 10.1016/s0197-0186(98)00068-0.