PMID- 10099934
OWN - NLM
STAT- MEDLINE
DCOM- 19990707
LR  - 20181113
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 78
IP  - 2
DP  - 1999 Feb
TI  - 2E4 (kaptin): a novel actin-associated protein from human blood platelets found
      in lamellipodia and the tips of the stereocilia of the inner ear.
PG  - 117-26
AB  - Platelet activation, crucial for hemostasis, requires actin polymerization, yet
      the molecular mechanisms by which localized actin polymerization is mediated are 
      not clear. Here we report the characterization of a novel actin-binding protein, 
      2E4, originally isolated from human blood platelets and likely to be involved in 
      the actin rearrangements occurring during activation. 2E4 binds to filamentous
      (F)-actin by F-actin affinity chromatography and is eluted from F-actin affinity 
      columns and extracted from cells with ATP. Its presence at the leading edge of
      platelets spread on glass and in the lamellipodia of motile fibroblasts suggests 
      a role in actin dynamics. Using localization to obtain clues about function, we
      stained the sensory epithelium of the embryonic inner ear to determine whether
      2E4 is at the barbed end of actin filaments during their elongation. Indeed, 2E4 
      was present at the tips of the elongating stereocilium. 2E4 is novel by DNA
      sequence and has no identifiable structural motifs. Its unusual amino acid
      sequence, its ATP-sensitive actin association and its location at sites of actin 
      polymerization in cells suggest 2E4 plays a unique role in the actin
      rearrangements that accompany platelet activation and stereocilia formation.
FAU - Bearer, E L
AU  - Bearer EL
AD  - Department of Pathology and Laboratory Medicine, Brown University, Providence, RI
      02912, USA. Elaine_Bearer@Brown.edu
FAU - Abraham, M T
AU  - Abraham MT
LA  - eng
SI  - GENBANK/AF105369
GR  - R01 GM047368/GM/NIGMS NIH HHS/United States
GR  - R01 GM047368-05/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Actins)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (KPTN protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Actins/metabolism
MH  - Adenosine Triphosphate/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Blood Platelets/chemistry/*metabolism
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Chromatography, Affinity
MH  - Cloning, Molecular
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fibroblasts/metabolism
MH  - Fluorescent Antibody Technique
MH  - Hair Cells, Auditory/*metabolism
MH  - Humans
MH  - Intestinal Mucosa/metabolism
MH  - Mice
MH  - Microfilament Proteins/chemistry/genetics/immunology/*metabolism
MH  - Molecular Sequence Data
MH  - Pseudopodia/*metabolism
PMC - PMC3376092
MID - NIHMS242051
EDAT- 1999/04/01 00:00
MHDA- 1999/04/01 00:01
CRDT- 1999/04/01 00:00
PHST- 1999/04/01 00:00 [pubmed]
PHST- 1999/04/01 00:01 [medline]
PHST- 1999/04/01 00:00 [entrez]
AID - S0171-9335(99)80013-2 [pii]
AID - 10.1016/S0171-9335(99)80013-2 [doi]
PST - ppublish
SO  - Eur J Cell Biol. 1999 Feb;78(2):117-26. doi: 10.1016/S0171-9335(99)80013-2.