PMID- 10098881
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 72
IP  - 4
DP  - 1999 Apr
TI  - Cellular distribution and developmental expression of AMP-activated protein
      kinase isoforms in mouse central nervous system.
PG  - 1707-16
AB  - The mammalian AMP-activated protein kinase is a heterotrimeric serine/threonine
      protein kinase with multiple isoforms for each subunit (alpha, beta, and gamma)
      and is activated under conditions of metabolic stress. It is widely expressed in 
      many tissues, including the brain, although its expression pattern throughout the
      CNS is unknown. We show that brain mRNA levels for the alpha2 and beta2 subunits 
      were increased between embryonic days 10 and 14, whereas expression of alpha1,
      beta1, and gamma1 subunits was consistent at all ages examined. Immunostaining
      revealed a mainly neuronal distribution of all isoforms. The alpha2 catalytic
      subunit was highly expressed in neurons and activated astrocytes, whereas the
      alpha1 catalytic subunit showed low expression in neuropil. The gamma1
      noncatalytic subunit was highly expressed by neurons, but not by astrocytes.
      Expression of the beta1 and beta2 noncatalytic subunits varied, but some neurons,
      such as granule cells of olfactory bulb, did not express detectable levels of
      either beta isoform. Preferential nuclear localization of the alpha2, beta1, and 
      gamma1 subunits suggests new functions of the AMP-activated protein kinase, and
      the different expression patterns and cellular localization between the two
      catalytic subunits alpha1 and alpha2 point to different physiological roles.
FAU - Turnley, A M
AU  - Turnley AM
AD  - The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne
      Hospital, Victoria, Australia.
FAU - Stapleton, D
AU  - Stapleton D
FAU - Mann, R J
AU  - Mann RJ
FAU - Witters, L A
AU  - Witters LA
FAU - Kemp, B E
AU  - Kemp BE
FAU - Bartlett, P F
AU  - Bartlett PF
LA  - eng
GR  - DK35712/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Antibodies)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.4.3 (Adenylate Kinase)
SB  - IM
MH  - Adenylate Kinase/analysis/*genetics/immunology
MH  - Age Factors
MH  - Animals
MH  - Antibodies
MH  - Astrocytes/enzymology
MH  - Cell Nucleus/enzymology
MH  - Central Nervous System/cytology/*enzymology/*growth & development
MH  - *Gene Expression Regulation, Developmental
MH  - *Gene Expression Regulation, Enzymologic
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Neurons/enzymology
MH  - RNA, Messenger/analysis
EDAT- 1999/03/31 00:00
MHDA- 1999/03/31 00:01
CRDT- 1999/03/31 00:00
PHST- 1999/03/31 00:00 [pubmed]
PHST- 1999/03/31 00:01 [medline]
PHST- 1999/03/31 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.721707.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Apr;72(4):1707-16. doi: 10.1046/j.1471-4159.1999.721707.x.