PMID- 10097072
OWN - NLM
STAT- MEDLINE
DCOM- 19990512
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 7
DP  - 1999 Mar 30
TI  - Tumor necrosis factor receptor family member RANK mediates osteoclast
      differentiation and activation induced by osteoprotegerin ligand.
PG  - 3540-5
AB  - A receptor that mediates osteoprotegerin ligand (OPGL)-induced osteoclast
      differentiation and activation has been identified via genomic analysis of a
      primary osteoclast precursor cell cDNA library and is identical to the tumor
      necrosis factor receptor (TNFR) family member RANK. The RANK mRNA was highly
      expressed by isolated bone marrow-derived osteoclast progenitors and by mature
      osteoclasts in vivo. Recombinant OPGL binds specifically to RANK expressed by
      transfected cell lines and purified osteoclast progenitors. Transgenic mice
      expressing a soluble RANK-Fc fusion protein have severe osteopetrosis because of 
      a reduction in osteoclasts, similar to OPG transgenic mice. Recombinant RANK-Fc
      binds with high affinity to OPGL in vitro and blocks osteoclast differentiation
      and activation in vitro and in vivo. Furthermore, polyclonal Ab against the RANK 
      extracellular domain promotes osteoclastogenesis in bone marrow cultures
      suggesting that RANK activation mediates the effects of OPGL on the osteoclast
      pathway. These data indicate that OPGL-induced osteoclastogenesis is directly
      mediated through RANK on osteoclast precursor cells.
FAU - Hsu, H
AU  - Hsu H
AD  - Department of Cell Biology, Amgen, Inc., One Amgen Center Drive, Thousand Oaks,
      CA 91320-1799, USA.
FAU - Lacey, D L
AU  - Lacey DL
FAU - Dunstan, C R
AU  - Dunstan CR
FAU - Solovyev, I
AU  - Solovyev I
FAU - Colombero, A
AU  - Colombero A
FAU - Timms, E
AU  - Timms E
FAU - Tan, H L
AU  - Tan HL
FAU - Elliott, G
AU  - Elliott G
FAU - Kelley, M J
AU  - Kelley MJ
FAU - Sarosi, I
AU  - Sarosi I
FAU - Wang, L
AU  - Wang L
FAU - Xia, X Z
AU  - Xia XZ
FAU - Elliott, R
AU  - Elliott R
FAU - Chiu, L
AU  - Chiu L
FAU - Black, T
AU  - Black T
FAU - Scully, S
AU  - Scully S
FAU - Capparelli, C
AU  - Capparelli C
FAU - Morony, S
AU  - Morony S
FAU - Shimamoto, G
AU  - Shimamoto G
FAU - Bass, M B
AU  - Bass MB
FAU - Boyle, W J
AU  - Boyle WJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Carrier Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TNFRSF11A protein, human)
RN  - 0 (TNFRSF11B protein, human)
RN  - 0 (TNFSF11 protein, human)
RN  - 0 (Tnfrsf11a protein, mouse)
RN  - 0 (Tnfrsf11b protein, mouse)
RN  - 0 (Tnfsf11 protein, mouse)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Bone Development
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - *Carrier Proteins
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cloning, Molecular
MH  - *Gene Expression Regulation, Developmental
MH  - Glycoproteins/*pharmacology/physiology
MH  - Humans
MH  - Immunoglobulin G
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Male
MH  - *Membrane Glycoproteins
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - *Mitogen-Activated Protein Kinases
MH  - Osteoclasts/*cytology/drug effects
MH  - Osteogenesis
MH  - Osteoprotegerin
MH  - RANK Ligand
MH  - Receptor Activator of Nuclear Factor-kappa B
MH  - *Receptors, Cytoplasmic and Nuclear
MH  - Receptors, Tumor Necrosis Factor/genetics/*physiology
MH  - Recombinant Fusion Proteins/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Signal Transduction
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC22329
EDAT- 1999/03/31 00:00
MHDA- 1999/03/31 00:01
CRDT- 1999/03/31 00:00
PHST- 1999/03/31 00:00 [pubmed]
PHST- 1999/03/31 00:01 [medline]
PHST- 1999/03/31 00:00 [entrez]
AID - 10.1073/pnas.96.7.3540 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3540-5. doi: 10.1073/pnas.96.7.3540.