PMID- 10095772
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 260
IP  - 2
DP  - 1999 Mar
TI  - Identification of cyclin A/Cdk2 phosphorylation sites in B-Myb.
PG  - 384-91
AB  - B-myb is a highly conserved member of the myb proto-oncogene family that encodes 
      a ubiquitously expressed 110-kDa sequence-specific DNA-binding protein.
      Transactivation of Myb-inducible promoters by B-Myb is repressed by a regulatory 
      domain located at the C-terminus of the protein. Cyclin A/Cdk2-mediated
      phosphorylation apparently releases the negative constraint and triggers B-Myb
      transactivation potential. Two-dimensional tryptic phosphopeptide analysis
      indicated that the majority of the sites phosphorylated in vivo are targeted in
      vitro by cyclin A/Cdk2. Six sites in B-Myb fulfil the requirements for
      recognition by Cdk2. Using point mutation of the phosphorylation sites to
      nonphosphorylatable amino acids, we show that five of these sites are targets for
      Cdk2 in vivo. Mutation of one of these residues (T524) to alanine diminished the 
      ability of B-Myb to promote transcription of a reporter gene, suggesting that
      phosphorylation of B-Myb at this site is important for the regulation of its
      activity by cyclin A/Cdk2.
FAU - Bartsch, O
AU  - Bartsch O
AD  - Institute for Experimental Cancer Research, Tumor Biology Center, Freiburg,
      Germany.
FAU - Horstmann, S
AU  - Horstmann S
FAU - Toprak, K
AU  - Toprak K
FAU - Klempnauer, K H
AU  - Klempnauer KH
FAU - Ferrari, S
AU  - Ferrari S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin A)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MYBL2 protein, human)
RN  - 0 (Trans-Activators)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (CDK2 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Binding Sites/genetics
MH  - *CDC2-CDC28 Kinases
MH  - *Cell Cycle Proteins
MH  - Cells, Cultured
MH  - Cyclin A/*metabolism
MH  - Cyclin-Dependent Kinase 2
MH  - Cyclin-Dependent Kinases/*metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Humans
MH  - Phosphorylation
MH  - Point Mutation
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Quail
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcriptional Activation
MH  - Transfection
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00191.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Mar;260(2):384-91. doi: 10.1046/j.1432-1327.1999.00191.x.