PMID- 10095771
OWN - NLM
STAT- MEDLINE
DCOM- 19990426
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 260
IP  - 2
DP  - 1999 Mar
TI  - Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical
      isolate of Burkholderia cepacia. Characterization of a trisubstituted glucuronic 
      acid residue in a heptasaccharide repeating unit.
PG  - 373-83
AB  - The structure of the exopolysaccharide (EPS) produced by a clinical isolate of
      Burkholderia cepacia isolated from a patient with fibrocystic lung disease has
      been investigated. By means of methylation analyses, carboxyl reduction, partial 
      depolymerization by fuming HCl and chemical degradations such as Smith
      degradation, lithiumethylenediamine degradation and beta-elimination, supported
      by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been
      identified and was shown to correspond to the acidic branched heptasaccharide
      with the following structure: [formula: see text]. This partially acetylated
      acidic polymer, distinguished by the presence of the less usual D-isomer of
      rhamnose and of a trisubstituted glucuronic acid residue, could represent the
      main EPS produced by this bacterial species.
FAU - Cerantola, S
AU  - Cerantola S
AD  - Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France.
      cerant@ipbs.fr
FAU - Lemassu-Jacquier, A
AU  - Lemassu-Jacquier A
FAU - Montrozier, H
AU  - Montrozier H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Glucuronates)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (polysaccharide II, Burkholderia)
RN  - 8A5D83Q4RW (Glucuronic Acid)
SB  - IM
MH  - Burkholderia Infections/*microbiology
MH  - Burkholderia cepacia/chemistry/*metabolism
MH  - Carbohydrate Sequence
MH  - Gas Chromatography-Mass Spectrometry
MH  - Glucuronates/*analysis
MH  - Glucuronic Acid
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - Polysaccharides, Bacterial/*chemistry/isolation & purification
MH  - Pulmonary Fibrosis/*microbiology
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00171.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Mar;260(2):373-83. doi: 10.1046/j.1432-1327.1999.00171.x.