PMID- 10095107
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 229
IP  - 1-2
DP  - 1999 Mar 18
TI  - Cloning, expression and chromosomal localization of a human testis
      6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene.
PG  - 83-9
AB  - 6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a
      bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2, a 
      key metabolite in the regulation of glycolysis. Several genes encode distinct
      PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme
      regulation. In this paper, we present the isolation of a cDNA from a human testis
      cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open 
      reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids.
      This has a calculated molecular weight of 54kDa and 97% similarity with rat
      testis PFK-2/FBPase-2, with complete conservation of the amino acid residues
      involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH)
      localized testis PFK-2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands
      p21-p22. A Northern blot analysis of different rat tissues showed the presence of
      a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the
      human testis cDNA drives expression of an isozyme with a molecular weight of
      55kDa. This isozyme shows clear PFK-2 activity. Taken together, these results
      provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme.
FAU - Manzano, A
AU  - Manzano A
AD  - Unitat de Bioquimica, Campus de Bellvitge, U.B.C/Feixa Llarga sn E-08907,
      L'Hospitalet, Spain.
FAU - Perez, J X
AU  - Perez JX
FAU - Nadal, M
AU  - Nadal M
FAU - Estivill, X
AU  - Estivill X
FAU - Lange, A
AU  - Lange A
FAU - Bartrons, R
AU  - Bartrons R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Isoenzymes)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.105 (Phosphofructokinase-2)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 3.1.3.11 (Fructose-Bisphosphatase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3
MH  - Cloning, Molecular
MH  - Fructose-Bisphosphatase/*genetics
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Isoenzymes/genetics
MH  - Liver/enzymology
MH  - Male
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/*genetics
MH  - Myocardium/enzymology
MH  - Phosphofructokinase-1/*genetics
MH  - Phosphofructokinase-2
MH  - RNA, Messenger/metabolism
MH  - Sequence Alignment
MH  - Testis/*enzymology
MH  - Transfection
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - S0378-1119(99)00037-2 [pii]
AID - 10.1016/s0378-1119(99)00037-2 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 18;229(1-2):83-9. doi: 10.1016/s0378-1119(99)00037-2.