PMID- 10095106
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 229
IP  - 1-2
DP  - 1999 Mar 18
TI  - TRAG-3, a novel gene, isolated from a taxol-resistant ovarian carcinoma cell
      line.
PG  - 75-81
AB  - The mechanisms responsible for the development of the taxol resistance phenotype 
      are unclear, and are likely explained by multiple mechanisms. To understand the
      molecular changes associated with drug resistance more fully, a taxol-resistant
      subline, derived from the human ovarian cancer cell line SKOV-3, was established 
      through selection by culture in incrementally increasing taxol concentrations.
      Comparison of SKOV-3 to SKOV-3TR by differential display identifies a new gene,
      TRAG-3 (Taxol Resistance Associated Gene- 3). In comparison to the parental line,
      SKOV-3, TRAG-3 mRNA is overexpressed in the taxol-resistant cell line SKOV-3TR.
      The nucleotide sequence of the TRAG-3 cDNA contains an open reading frame of
      333bp that predicts for a protein product of 110 amino acids. A GenBank search
      identifies a cosmid clone containing a genomic sequence corresponding to that of 
      TRAG-3. DNA and protein analysis reveals that TRAG-3 has no homology to any known
      cDNAs or proteins. Northern analysis demonstrates that TRAG-3 is overexpressed in
      the taxol-resistant breast cancer cell line MDA 435TR as well as the
      doxorubicin-resistant multiple myeloma cell lines 8226/DOX40 and 8226/MDR10V. A
      survey of normal tissue shows minimal or absent TRAG-3 mRNA expression. Screening
      of a wide variety of cancer cell lines demonstrates TRAG-3 expression in many
      cell lines derived from different tissue types. In summary, TRAG-3 is a novel
      gene whose expression is associated with the chemotherapy-resistant and
      neoplastic phenotype.
FAU - Duan, Z
AU  - Duan Z
AD  - Masssachusetts General Hospital, Cox 640, Department of Hematology/Oncology, 100 
      Blossom Street, Boston, MA 02114, USA.
FAU - Feller, A J
AU  - Feller AJ
FAU - Toh, H C
AU  - Toh HC
FAU - Makastorsis, T
AU  - Makastorsis T
FAU - Seiden, M V
AU  - Seiden MV
LA  - eng
SI  - GENBANK/AF080246
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (CSAG2 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 80168379AG (Doxorubicin)
RN  - P88XT4IS4D (Paclitaxel)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Doxorubicin/metabolism
MH  - Drug Resistance/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, Neoplasm
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/chemistry/*genetics
MH  - Ovarian Neoplasms/genetics
MH  - Paclitaxel/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - S0378-1119(99)00042-6 [pii]
AID - 10.1016/s0378-1119(99)00042-6 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 18;229(1-2):75-81. doi: 10.1016/s0378-1119(99)00042-6.