PMID- 10095098
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 229
IP  - 1-2
DP  - 1999 Mar 18
TI  - Genomic sequence and structural organization of mouse slow skeletal muscle
      troponin T gene.
PG  - 1-10
AB  - Three muscle type-specific troponin T (TnT) genes are present in vertebrate to
      encode a number of protein isoforms via alternative mRNA splicing. While the
      genomic structures of cardiac and fast skeletal muscle TnT genes have been
      documented, this study cloned and characterized the slow skeletal muscle TnT
      (sTnT) gene. Complete nucleotide sequence and genomic organization revealed that 
      the mouse sTnT gene spans 11.1kb and contains 14 exons, which is smaller and
      simpler than the fast skeletal muscle and cardiac TnT genes. Potentially
      representing a prototype of the TnT gene family, the 5'-region of the sTnT gene
      contains fewer unsplit large exons, among which two alternatively spliced exons
      are responsible for the NH2-terminal variation of three sTnT isoforms. The sTnT
      gene structure shows that the alternatively spliced central segment found in
      human sTnT cDNAs may be a result from splicing using an alternative acceptor site
      at the intron 11-exon 12 boundary. Together with the well-conserved protein
      structure, the highly specific expression of sTnT in slow skeletal muscles
      indicates a differentiated function of this member of the TnT gene family. The
      determination of genomic structure and alternative splicing pathways of sTnT gene
      lays a foundation to further understand the TnT structure-function evolution as
      well as contractile characteristics of different types of muscle fiber.
FAU - Huang, Q Q
AU  - Huang QQ
AD  - Department of Physiology and Biophysics, Case Western Reserve University School
      of Medicine, 10900 Euclid Ave., Cleveland, OH 44106-4970, USA.
FAU - Chen, A
AU  - Chen A
FAU - Jin, J P
AU  - Jin JP
LA  - eng
SI  - GENBANK/U92882
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Troponin T)
SB  - IM
SB  - S
MH  - Alternative Splicing/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Introns/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Muscle Contraction/genetics
MH  - Muscle Fibers, Slow-Twitch/metabolism
MH  - Muscle, Skeletal/*metabolism
MH  - Protein Isoforms/genetics
MH  - RNA, Messenger/genetics
MH  - Troponin T/*genetics
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - S0378-1119(99)00051-7 [pii]
AID - 10.1016/s0378-1119(99)00051-7 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 18;229(1-2):1-10. doi: 10.1016/s0378-1119(99)00051-7.