PMID- 10095079
OWN - NLM
STAT- MEDLINE
DCOM- 19990513
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 66
IP  - 1-2
DP  - 1999 Mar 20
TI  - Developmental changes in nicotinic receptor mRNAs and responses to nicotine in
      the suprachiasmatic nucleus and other brain regions.
PG  - 71-82
AB  - Our previous studies demonstrated that nicotine induces c-fos expression in the
      suprachiasmatic nucleus (SCN) of the rat during a narrow developmental window
      occurring in the perinatal period. We have extended these observations by showing
      that c-fos cannot be induced in the adult SCN by nicotine even during the
      subjective night, when phase shifts do occur. In contrast to the SCN, significant
      induction of c-fos and NGFI-A was observed in the medial habenula and
      paraventricular nucleus at all circadian times. In the fetal rat SCN we show that
      NGFI-A and junB are also induced by nicotine, but not c-jun. To investigate
      whether changes in nicotinic acetylcholine receptor (nAChR) expression in the SCN
      may underlie this change in sensitivity during the perinatal period, we examined 
      nAChR mRNAs across this developmental period. By Northern analyses, alpha2,
      alpha3 and alpha4 subunit mRNAs are relatively abundant in the fetal SCN but
      decline substantially in the adult. alpha7 mRNA increases substantially while
      beta2 mRNA is relatively abundant throughout development. We also examine
      expression in the whole mouse brain beginning at embryonic day 11. Many mRNA
      sizes for nAChR subunits in both the rat and mouse are characterized here for the
      first time by Northern analyses and some show very large changes in expression
      across development. In particular, a small 1.4 kb alpha2-related mRNA is highly
      expressed during early development, perhaps indicating an important novel
      function for this subunit.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - O'Hara, B F
AU  - O'Hara BF
AD  - Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, 
      USA. bfo@leland.stanford.edu
FAU - Macdonald, E
AU  - Macdonald E
FAU - Clegg, D
AU  - Clegg D
FAU - Wiler, S W
AU  - Wiler SW
FAU - Andretic, R
AU  - Andretic R
FAU - Cao, V H
AU  - Cao VH
FAU - Miller, J D
AU  - Miller JD
FAU - Heller, H C
AU  - Heller HC
FAU - Kilduff, T S
AU  - Kilduff TS
LA  - eng
GR  - AG11084/AG/NIA NIH HHS/United States
GR  - DA00187/DA/NIDA NIH HHS/United States
GR  - HD29732/HD/NICHD NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nicotinic)
RN  - 6M3C89ZY6R (Nicotine)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/physiology
MH  - Animals
MH  - Blotting, Northern
MH  - Circadian Rhythm/physiology
MH  - DNA Probes
MH  - DNA, Complementary
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Genes, Immediate-Early/physiology
MH  - Habenula/chemistry/cytology
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/chemistry/physiology
MH  - Nicotine/*pharmacology
MH  - Nicotinic Agonists/*pharmacology
MH  - Paraventricular Hypothalamic Nucleus/chemistry/cytology
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - Proto-Oncogene Proteins c-jun/genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Nicotinic/*genetics
MH  - Suprachiasmatic Nucleus/*chemistry/cytology
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - S0169-328X(99)00004-2 [pii]
AID - 10.1016/s0169-328x(99)00004-2 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Mar 20;66(1-2):71-82. doi:
      10.1016/s0169-328x(99)00004-2.