PMID- 10095065
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1444
IP  - 3
DP  - 1999 Mar 19
TI  - Molecular cloning and biochemical characterization of a truncated, secreted
      member of the human family of Ca2+-activated Cl- channels.
PG  - 418-23
AB  - A novel family of chloride channel proteins has recently been discovered
      including two bovine (Lu-ECAM-1, bCLCA1), one murine (mCLCA1), and two human
      (hCLCA1 and hCLCA2) members. Here, we describe the cloning, expression, and
      molecular characterization of a truncated human homolog, tentatively named
      hCLCA3. It was cloned from a human spleen cDNA library and is expressed in
      numerous tissues including lung, trachea, spleen, thymus, and mammary gland as
      determined by reverse transcriptase-polymerase chain reaction. Unlike all
      previously known CLCA family members which consistently encode an approximately
      125-kDa transmembrane protein that is cleaved to form a heterodimer of two
      proteins of approximately 90 and 35 kDa, the 3.6-kb hCLCA3 mRNA encodes a 37-kDa 
      glycoprotein that corresponds to the N-terminal extracellular domain of its
      homologs. Moreover, when expressed in human embryonic kidney 293 or Chinese
      hamster ovary cells, this 37-kDa glycoprotein is secreted into the culture
      supernatant. These observations suggest that hCLCA3 is a structurally divergent
      member of the CLCA family of proteins and that it does not act as a channel
      protein but has distinct, yet unidentified functions.
FAU - Gruber, A D
AU  - Gruber AD
AD  - Department of Molecular Medicine, Cancer Biology Laboratories, College of
      Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
FAU - Pauli, B U
AU  - Pauli BU
LA  - eng
SI  - GENBANK/AF043976
GR  - CA47668/CA/NCI NIH HHS/United States
GR  - CA71626/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (CLCA3P protein, human)
RN  - 0 (Chloride Channels)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Chloride Channels
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Humans
MH  - Membrane Glycoproteins/biosynthesis/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Sequence Alignment
EDAT- 1999/03/30 00:00
MHDA- 1999/03/30 00:01
CRDT- 1999/03/30 00:00
PHST- 1999/03/30 00:00 [pubmed]
PHST- 1999/03/30 00:01 [medline]
PHST- 1999/03/30 00:00 [entrez]
AID - S0167-4781(99)00008-1 [pii]
AID - 10.1016/s0167-4781(99)00008-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Mar 19;1444(3):418-23. doi:
      10.1016/s0167-4781(99)00008-1.