PMID- 10095061
OWN - NLM
STAT- MEDLINE
DCOM- 19990506
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1444
IP  - 3
DP  - 1999 Mar 19
TI  - memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the 
      metastasizing human melanoma cell lines BLM and MV3.
PG  - 384-94
AB  - memA was isolated by subtractive hybridization in which the mRNA repertoire was
      compared in a panel of human melanoma cell lines with different metastasizing
      potential. Expression of memA mRNA is elevated in the highly metastasizing human 
      melanoma cell lines and derived xenografts, as compared with the
      non-metastasizing ones. In a collection of human tumor cell lines and melanoma
      metastasis lesions, memA mRNA expression could be detected in the A-431
      (epidermoid carcinoma), HT-1080 (fibrosarcoma), JEG-3 and JAR (choriocarcinomas) 
      cell lines and in three out of 11 melanoma metastasis lesions. The distribution
      of memA mRNA in a collection of healthy human organs is also tissue restricted.
      Sequence analysis revealed that the MEMA protein is identical with a 160 kDa
      nuclear 'domain rich in serines' (DRS) protein occurring free in the nucleoplasm 
      and in U2-ribonucleoprotein structures. MEMA is also homologous to pinin, a 140
      kDa protein associated with the desmosome-intermediate filament complex, and to a
      32 kDa porcine neutrophilic protein that was copurified with components of the
      NADPH-oxidase enzyme complex. The encoded amino acid sequence predicts that the
      MEMA protein has three coiled-coil domains, one glycine loop domain, is very
      hydrophilic and contains regions rich in glutamine/proline, glutamic acid and
      serine residues.
FAU - Degen, W G
AU  - Degen WG
AD  - Department of Biochemistry 161, University of Nijmegen, P.O. Box 9101, NL-6500
      HB, Nijmegen, The Netherlands.
FAU - Agterbos, M A
AU  - Agterbos MA
FAU - Muyrers, J P
AU  - Muyrers JP
FAU - Bloemers, H P
AU  - Bloemers HP
FAU - Swart, G W
AU  - Swart GW
LA  - eng
SI  - GENBANK/Y09703
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PNN protein, human)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - *Cell Adhesion Molecules
MH  - Cloning, Molecular
MH  - Humans
MH  - Kidney/metabolism
MH  - Lung/metabolism
MH  - Melanoma/genetics
MH  - Molecular Sequence Data
MH  - Neoplasm Metastasis
MH  - Nuclear Proteins/analysis/biosynthesis/*genetics
MH  - RNA, Messenger/*analysis/biosynthesis
MH  - Sequence Alignment
MH  - Skin Neoplasms/genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/03/30 03:02
MHDA- 2000/05/20 09:00
CRDT- 1999/03/30 03:02
PHST- 1999/03/30 03:02 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 1999/03/30 03:02 [entrez]
AID - S0167-4781(99)00012-3 [pii]
AID - 10.1016/s0167-4781(99)00012-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Mar 19;1444(3):384-94. doi:
      10.1016/s0167-4781(99)00012-3.