PMID- 10094485
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 445
IP  - 2-3
DP  - 1999 Feb 26
TI  - Cloning and expression of interleukin-18 binding protein.
PG  - 338-42
AB  - Interleukin-18 binding protein is a novel glycoprotein that we successfully
      cloned and expressed. First, murine interleukin-18 binding protein was purified
      from the sera of mice with endotoxin shock using ligand affinity chromatography. 
      The murine interleukin-18 binding protein cDNA was cloned after RT-PCR using
      mixed primer pair sequences based on partial murine interleukin-18 binding
      protein amino acid sequence analysis. Subsequently, human interleukin-18 binding 
      protein cDNA was cloned from cDNA libraries of normal human liver using murine
      interleukin-18 binding protein cDNA as a probe. Next, we transiently expressed
      recombinant human and murine interleukin-18 binding proteins in COS-1 cells and
      purified them from culture supernatants. Both recombinant interleukin-18 binding 
      proteins did not exhibit species specificity and prevented interleukin-18 binding
      to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma 
      production from KG-1 cells effectively. These results suggest that the
      interleukin-18 binding protein may possess interleukine-18 antagonist activity.
FAU - Aizawa, Y
AU  - Aizawa Y
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories, Okayama, Japan.
      fujisaki@hayashibara.co.jp
FAU - Akita, K
AU  - Akita K
FAU - Taniai, M
AU  - Taniai M
FAU - Torigoe, K
AU  - Torigoe K
FAU - Mori, T
AU  - Mori T
FAU - Nishida, Y
AU  - Nishida Y
FAU - Ushio, S
AU  - Ushio S
FAU - Nukada, Y
AU  - Nukada Y
FAU - Tanimoto, T
AU  - Tanimoto T
FAU - Ikegami, H
AU  - Ikegami H
FAU - Ikeda, M
AU  - Ikeda M
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
SI  - GENBANK/AB019504
SI  - GENBANK/AB019505
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Proteins)
RN  - 0 (interleukin-18 binding protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - COS Cells
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Gene Expression
MH  - Glycoproteins/*genetics/metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Interleukin-18/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proteins/*genetics/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - S0014-5793(99)00148-9 [pii]
AID - 10.1016/s0014-5793(99)00148-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Feb 26;445(2-3):338-42. doi: 10.1016/s0014-5793(99)00148-9.