PMID- 10094400
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20181113
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 191
IP  - 1-2
DP  - 1999 Jan
TI  - BTF3 is a potential new substrate of protein kinase CK2.
PG  - 121-8
AB  - BTF3, initially discovered as a factor required for transcription inititation of 
      RNA polymerase II, is expressed in two isoforms, termed a and b. BTF3b, the
      transcriptionally inactive isoform, was identified as an interaction partner of
      protein kinase CK2 subunit beta employing the interaction trap system for
      screening ofa HeLa cDNA fusion library. We report here on the interaction between
      the other isoform, BTF3a, and protein kinase CK2. The complete cDNA of BTF3a was 
      cloned by RT-PCR and used for analysis in the two-hybrid system with a
      three-reporter yeast strain. Interaction of BTF3a with CK2 subunits alpha, alpha'
      or beta was detectable by one of three reporters, whereas the CK2beta - BTF3a
      interaction was activating two reporters. It was also shown that BTF3a is
      phosphorylated in vitro by the alpha2beta2 holoenzyme, but not by alpha or alpha'
      alone, indicating the requirement of beta for substrate recognition.
      Immunoprecipitations of GST-fused BTF3a carried out in vitro resulted in
      co-precipitation of beta. Similarly, GST-BTF3a, but not GST alone isolated with
      glutathione agarose beads from buffer containing recombinant CK2 subunits was
      found complexed with alpha and beta, likely representing alpha2beta2 holoenzyme. 
      The data show a weak, nevertheless specific interaction of protein kinase CK2 via
      subunit beta with the putative transcription factor BTF3a in vitro and in vivo,
      and a role of BTF3a as a potential new substrate for CK2.
FAU - Grein, S
AU  - Grein S
AD  - Biochemische Zellphysiologie, Deutsches Krebsforschungszentrum, Heidelberg,
      Germany.
FAU - Pyerin, W
AU  - Pyerin W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (DNA Primers)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (transcription factor BTF3)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Casein Kinase II
MH  - DNA Primers
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Substrate Specificity
MH  - Transcription Factors/chemistry/*metabolism
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
PST - ppublish
SO  - Mol Cell Biochem. 1999 Jan;191(1-2):121-8.