PMID- 10094309
OWN - NLM
STAT- MEDLINE
DCOM- 19990419
LR  - 20190607
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 5
IP  - 3
DP  - 1999 Mar
TI  - Cloning and characterization of a mammalian pseudouridine synthase.
PG  - 409-19
AB  - This report describes the cloning and characterization of a pseudouridine (psi)
      synthase from mouse that we have named mouse pseudouridine synthase 1 (mpus1p).
      The cDNA is approximately 1.5 kb and when used as a probe on a Northern blot of
      mouse RNA from tissues and cultured cells, several bands were detected. The open 
      reading frame is 393 amino acids and has 35% identity over its length with yeast 
      psi synthase 1 (pus1p). The recombinant protein was expressed in Escherichia coli
      and the purified protein converted specific uridines to psi in a number of tRNA
      substrates. The positions modified in stoichiometric amounts in vitro were 27/28 
      in the anticodon stem and also positions 34 and 36 in the anticodon of an intron 
      containing tRNA. A human cDNA was also cloned and the smaller open reading frame 
      (348 amino acids) was 92% identical over its length with mpus1p but is shorter by
      45 amino acids at the amino terminus. The expressed recombinant human protein has
      no activity on any of the tRNA substrates, most probably the result of the
      truncated open reading frame.
FAU - Chen, J
AU  - Chen J
AD  - Department of Pathology, School of Medicine, University of South Carolina,
      Columbia 29208, USA.
FAU - Patton, J R
AU  - Patton JR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Recombinant Proteins)
RN  - 9014-25-9 (RNA, Transfer)
RN  - EC 3.1.- (Endoribonucleases)
RN  - EC 3.1.27.1 (ribonuclease T(2))
RN  - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)
RN  - EC 4.2.1.- (Hydro-Lyases)
RN  - EC 4.2.1.70 (pseudouridylate synthetase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Endoribonucleases/metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/genetics
MH  - Humans
MH  - Hydro-Lyases/*biosynthesis/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - RNA, Transfer/chemistry/genetics
MH  - Recombinant Proteins/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Single-Strand Specific DNA and RNA Endonucleases/metabolism
MH  - Substrate Specificity
PMC - PMC1369769
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1017/s1355838299981591 [doi]
PST - ppublish
SO  - RNA. 1999 Mar;5(3):409-19. doi: 10.1017/s1355838299981591.