PMID- 10092875
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 259
IP  - 3
DP  - 1999 Feb
TI  - Chemotaxin-dependent translocation of immunoreactive ADP-ribosyltransferase-1 to 
      the surface of human neutrophil polymorphs.
PG  - 866-71
AB  - mRNA from human polymorphonuclear neutrophil leucocytes (PMNs) was probed with
      cDNA encoding human skeletal muscle arginine-specific ADP-ribosyltransferase
      (ART1). A single 2.6-kb transcript was identified, which was similar in size to
      that observed in human skeletal muscle RNA. An 872-bp cDNA fragment,
      corresponding to the amino acid sequence of the processed human skeletal muscle
      enzyme, was generated by reverse transcription-PCR amplification of RNA from
      human PMNs, and was found to be identical to the ART1 cDNA derived from human
      skeletal muscle. ART1 was expressed as a fusion protein with glutathione
      S-transferase (GST) in insect cells, and antibodies were raised against the
      fusion protein in a rabbit. Following removal of GST immunoreactivity by
      immunoprecipitation, these antibodies were used to measure the abundance of
      immunoreactive ART1 on the surface of PMNs. Exposure of PMNs to
      formyl-Met-Leu-Phe (FMLP) was followed by a rapid increase in the abundance of
      cell surface ART1 (T1/2 = 1.9 min), and the concentration of FMLP for
      half-maximum response was 28.6 nM. Similar responses were observed after exposure
      of the cells to platelet-activating factor or interleukin-8, and we conclude that
      some of the effects of these chemotaxins are mediated by translocation of an
      intracellular pool of ART1 to its site of catalytic activity on the outer aspect 
      of the plasma membrane.
FAU - Kefalas, P
AU  - Kefalas P
AD  - Section on Clinical Pharmacology, Imperial College School of Medicine, London,
      UK.
FAU - Saxty, B
AU  - Saxty B
FAU - Yadollahi-Farsani, M
AU  - Yadollahi-Farsani M
FAU - MacDermot, J
AU  - MacDermot J
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Chemotactic Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 4.6.1.13 (Phosphatidylinositol Diacylglycerol-Lyase)
SB  - IM
MH  - ADP Ribose Transferases/immunology/*metabolism
MH  - Animals
MH  - Cell Membrane/enzymology
MH  - Chemotactic Factors/*pharmacology
MH  - DNA, Complementary/genetics
MH  - Fluorescence
MH  - Humans
MH  - Muscle, Skeletal/enzymology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*enzymology
MH  - Phosphatidylinositol Diacylglycerol-Lyase
MH  - RNA, Messenger/metabolism
MH  - Recombinant Fusion Proteins/genetics
MH  - Spodoptera/genetics
MH  - Type C Phospholipases/metabolism
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00114.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Feb;259(3):866-71. doi: 10.1046/j.1432-1327.1999.00114.x.