PMID- 10092871
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 259
IP  - 3
DP  - 1999 Feb
TI  - N-glycan structures of matrix metalloproteinase-1 derived from human fibroblasts 
      and from HT-1080 fibrosarcoma cells.
PG  - 829-40
AB  - Matrix metalloproteinase-1 (MMP-1) is a collagenolytic metalloproteinase capable 
      of cleaving native triple-helical forms of several collagen subtypes, as well as 
      a number of non-collagenous substrates. The role of MMP-1 in various diseases
      affecting the connective tissue is well characterized. MMP-1 is secreted as both 
      glycosylated and unglycosylated species, and the two forms have been shown to be 
      identical with respect to substrate specificity, specific activity and inhibitory
      profile. No function for the glycan moiety of the enzyme has been ascribed to
      date. In the present study, we report on the detailed characterization of
      MMP-1-derived oligosaccharides. Using strategies based on sequential
      exoglycosidase digestion combined with matrix-assisted laser desorption
      ionization-time of flight MS and electrospray tandem MS, we have characterized
      the N-glycan structures of MMP-1, derived from human dermal fibroblasts and from 
      the HT-1080 fibrosarcoma cell line. MMP-1 derived from fibroblasts was found to
      carry mainly alpha 2,3-sialylated complex-type diantennary glycans. On the other 
      hand, HT-1080 cells produce MMP-1 that has a heterogeneous glycosylation pattern,
      comprising diantennary glycans carrying Lewis X, LacdiNAc, sialylated LacdiNAc
      and GalNAc beta 1,4 (Fuc alpha 1,3)GlcNAc (LacdiNAc analogue of Lewis X) as
      terminal elements. We also show that, of the two potential glycosylation sites in
      the MMP-1 sequence, only Asn120 is used.
FAU - Saarinen, J
AU  - Saarinen J
AD  - Institute of Biotechnology, University of Helsinki, Finland.
      juhani.saarinen@helsinki.fi
FAU - Welgus, H G
AU  - Welgus HG
FAU - Flizar, C A
AU  - Flizar CA
FAU - Kalkkinen, N
AU  - Kalkkinen N
FAU - Helin, J
AU  - Helin J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Oligosaccharides)
RN  - 0 (Polysaccharides)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Carbohydrate Sequence
MH  - Cells, Cultured
MH  - Collagenases/*chemistry
MH  - Glycoside Hydrolases/metabolism
MH  - Glycosylation
MH  - Humans
MH  - Mass Spectrometry
MH  - Matrix Metalloproteinase 1
MH  - Molecular Sequence Data
MH  - Oligosaccharides/chemistry
MH  - Polysaccharides/*chemistry
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00105.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Feb;259(3):829-40. doi: 10.1046/j.1432-1327.1999.00105.x.