PMID- 10092863
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 259
IP  - 3
DP  - 1999 Feb
TI  - Donor substrate specificity of recombinant human blood group A, B and hybrid A/B 
      glycosyltransferases expressed in Escherichia coli.
PG  - 770-5
AB  - The human blood group A and B glycosyltransferases catalyze the transfer of
      GalNAc and Gal, to the (O)H-precursor structure Fuc alpha (1-2)Gal beta-OR to
      form the blood group A and B antigens, respectively. Changing four amino acids
      (176, 235, 266 and 268) alters the specificity from an A to a B
      glycosyltransferase. A series of hybrid blood group A/B glycosyltransferases were
      produced by interchanging these four amino acids in synthetic genes coding for
      soluble forms of the enzymes and expressed in Escherichia coli. The purified
      hybrid glycosyltransferases were characterized by two-substrate enzyme kinetic
      analysis using both UDP-GalNAc and UDP-Gal donor substrates. The A and B
      glycosyltransferases were screened with other donor substrates and found to also 
      utilize the unnatural donors UDP-GlcNAc and UDP-Glc, respectively. The kinetic
      data demonstrate the importance of a single amino acid (266) in determining the A
      vs. B donor specificity.
FAU - Seto, N O
AU  - Seto NO
AD  - Institute for Biological Sciences, National Research Council of Canada, Ottawa,
      Ontario, Canada. nina.seto@nrc.ca
FAU - Compston, C A
AU  - Compston CA
FAU - Evans, S V
AU  - Evans SV
FAU - Bundle, D R
AU  - Bundle DR
FAU - Narang, S A
AU  - Narang SA
FAU - Palcic, M M
AU  - Palcic MM
LA  - eng
SI  - GENBANK/X91874
SI  - GENBANK/Y11891
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 2956-16-3 (Uridine Diphosphate Galactose)
RN  - 7277-98-7 (Uridine Diphosphate N-Acetylgalactosamine)
RN  - EC 2.4.1.- (Galactosyltransferases)
RN  - EC 2.4.1.- (N-Acetylgalactosaminyltransferases)
RN  - EC 2.4.1.- (UDPgalactosamine-galactose acetylgalactosaminyltransferase)
RN  - EC 2.4.1.37 (blood-group-substance alpha-D-galactosyltransferase)
SB  - IM
MH  - Carbohydrate Sequence
MH  - Escherichia coli/*genetics
MH  - Galactosyltransferases/genetics/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - N-Acetylgalactosaminyltransferases/genetics/*metabolism
MH  - Recombinant Fusion Proteins/chemistry/*genetics
MH  - Recombinant Proteins/genetics
MH  - Substrate Specificity
MH  - Uridine Diphosphate Galactose/metabolism
MH  - Uridine Diphosphate N-Acetylgalactosamine/metabolism
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00086.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Feb;259(3):770-5. doi: 10.1046/j.1432-1327.1999.00086.x.