PMID- 10092851
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 259
IP  - 3
DP  - 1999 Feb
TI  - Cloning and expression of the human p8, a nuclear protein with mitogenic
      activity.
PG  - 670-5
AB  - We have previously identified a new rat mRNA, provisionally named p8, which
      showed a strong, but transient, induction in the pancreas in response to acute
      pancreatitis. We report here the cloning and sequencing of the human p8 cDNA. The
      human p8 polypeptide is 82 amino acids long and shows an overall identity of 74% 
      with the rat counterpart. The complete structure of the p8 gene was also
      established. The p8 gene comprises three exons separated by two introns and the
      gene was mapped to chromosome 16. Analysis of the p8 primary structure suggested 
      the presence of a bipartite motif of nuclear targeting, indicating that p8 may
      function within the nucleus. This presumption has been confirmed by transfection 
      of COS-7 cells with the p8 cDNA followed by immunostaining with specific
      antibodies. p8 mRNA expression is induced in some, but not all, cells by serum
      starvation and ceramide. To analyze the p8 function, we stably transfected HeLa
      cells with a p8 expression plasmid, and measured their growth and their
      sensitivity to apoptosis. Response to TNF alpha and staurosporine-induced
      apoptosis was not modified by p8 expression. However, cells expressing p8 grew
      significantly more rapidly.
FAU - Vasseur, S
AU  - Vasseur S
AD  - U.315 INSERM, Marseille, France.
FAU - Vidal Mallo, G
AU  - Vidal Mallo G
FAU - Fiedler, F
AU  - Fiedler F
FAU - Bodeker, H
AU  - Bodeker H
FAU - Canepa, E
AU  - Canepa E
FAU - Moreno, S
AU  - Moreno S
FAU - Iovanna, J L
AU  - Iovanna JL
LA  - eng
SI  - GENBANK/AF069073
SI  - GENBANK/AF069074
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Growth Substances)
RN  - 0 (Mitogens)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (P8 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Apoptosis/drug effects/genetics
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cell Division/genetics
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - *DNA-Binding Proteins
MH  - Gene Expression/genetics
MH  - Growth Substances/biosynthesis/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Mitogens/*genetics
MH  - Molecular Sequence Data
MH  - *Neoplasm Proteins
MH  - Nuclear Proteins/*genetics
MH  - RNA, Messenger/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Staurosporine/pharmacology
MH  - Transfection/genetics
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1046/j.1432-1327.1999.00092.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Feb;259(3):670-5. doi: 10.1046/j.1432-1327.1999.00092.x.