PMID- 10092811
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20120528
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 6
DP  - 1999 Mar 15
TI  - Identification of a second major tumor-specific antigen recognized by CTLs on
      mouse mastocytoma P815.
PG  - 3534-40
AB  - Murine mastocytoma P815 induces CTL responses against at least four distinct Ags 
      (AB, C, D, and E). Recent studies have shown that the main component of the CTL
      response against the P815 tumor is targeted against Ags P815AB and P815E. The
      gene P1A has been well characterized. It encodes the P815AB Ag in the form of a
      nonameric peptide containing two epitopes, P815A and P815B, which are recognized 
      by different CTLs. Here, we report the identification of the P815E Ag. Using a
      cDNA library derived from tumor P815, we identified the gene coding for P815E. We
      also characterized the antigenic peptide that anti-P815E CTLs recognize on the
      MHC class I molecule H-2Kd. The P815E Ag results from a mutation within an
      ubiquitously expressed gene encoding methionine sulfoxide reductase, an enzyme
      that is believed to be important in the protection of proteins against the
      by-products of aerobic metabolism. Surprisingly, immunizing mice i.p. with
      syngeneic tumor cells (L1210) that were constructed to express B7-1 and P815E did
      not induce resistance against live P815, even though a strong anti-P815E CTL
      response was observed with splenocytes from immunized animals.
FAU - Bilsborough, J
AU  - Bilsborough J
AD  - Ludwig Institute for Cancer Research, Brussels Branch, Belgium.
FAU - Van Pel, A
AU  - Van Pel A
FAU - Uyttenhove, C
AU  - Uyttenhove C
FAU - Boon, T
AU  - Boon T
FAU - Van den Eynde, B J
AU  - Van den Eynde BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oligopeptides)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.8.4.- (Methionine Sulfoxide Reductases)
RN  - EC 1.8.4.11 (methionine sulfoxide reductase)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Neoplasm/genetics/immunology/*isolation & purification
MH  - Base Sequence
MH  - Cytotoxicity, Immunologic
MH  - DNA, Complementary/biosynthesis/isolation & purification
MH  - Epitopes, T-Lymphocyte/genetics/immunology/*isolation & purification
MH  - Immunization
MH  - Injections, Intraperitoneal
MH  - Mast-Cell Sarcoma/enzymology/*immunology/prevention & control
MH  - Methionine Sulfoxide Reductases
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/genetics/immunology/isolation & purification
MH  - Oligopeptides/genetics/immunology/isolation & purification
MH  - Oxidoreductases/genetics/immunology/*isolation & purification
MH  - T-Lymphocytes, Cytotoxic/enzymology/*immunology
MH  - Tumor Cells, Cultured
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Mar 15;162(6):3534-40.