PMID- 10092682
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 14
DP  - 1999 Apr 2
TI  - RGS7 and RGS8 differentially accelerate G protein-mediated modulation of K+
      currents.
PG  - 9899-904
AB  - The recently discovered family of RGS (regulators of G protein signaling)
      proteins acts as GTPase activating proteins which bind to alpha subunits of
      heterotrimeric G proteins. We previously showed that a brain-specific RGS, RGS8
      speeds up the activation and deactivation kinetics of the G protein-coupled
      inward rectifier K+ channel (GIRK) upon receptor stimulation (Saitoh, O., Kubo,
      Y., Miyatani, Y., Asano, T., and Nakata, H. (1997) Nature 390, 525-529). Here we 
      report the isolation of a full-length rat cDNA of another brain-specific RGS,
      RGS7. In situ hybridization study revealed that RGS7 mRNA is predominantly
      expressed in Golgi cells within granule cell layer of cerebellar cortex. We
      observed that RGS7 recombinant protein binds preferentially to Galphao, Galphai3,
      and Galphaz. When co-expressed with GIRK1/2 in Xenopus oocytes, RGS7 and RGS8
      differentially accelerate G protein-mediated modulation of GIRK. RGS7 clearly
      accelerated activation of GIRK current similarly with RGS8 but the acceleration
      effect of deactivation was significantly weaker than that of RGS8. These
      acceleration properties of RGS proteins may play important roles in the rapid
      regulation of neuronal excitability and the cellular responses to short-lived
      stimulations.
FAU - Saitoh, O
AU  - Saitoh O
AD  - Department of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute
      for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan.
      osaito@tmin.ac.jp
FAU - Kubo, Y
AU  - Kubo Y
FAU - Odagiri, M
AU  - Odagiri M
FAU - Ichikawa, M
AU  - Ichikawa M
FAU - Yamagata, K
AU  - Yamagata K
FAU - Sekine, T
AU  - Sekine T
LA  - eng
SI  - GENBANK/AB024398
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Potassium Channels)
RN  - 0 (Proteins)
RN  - 0 (RGS Proteins)
RN  - 0 (Rgs7 protein, mouse)
RN  - 0 (Rgs7 protein, rat)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - GTP Phosphohydrolases/metabolism
MH  - GTP-Binding Proteins/*physiology
MH  - GTPase-Activating Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Potassium Channels/*physiology
MH  - Proteins/*physiology
MH  - *RGS Proteins
MH  - Rats
MH  - Sequence Alignment
MH  - Signal Transduction
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1074/jbc.274.14.9899 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 2;274(14):9899-904. doi: 10.1074/jbc.274.14.9899.