PMID- 10092656
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 14
DP  - 1999 Apr 2
TI  - Interferon consensus sequence-binding protein is constitutively expressed and
      differentially regulated in the ocular lens.
PG  - 9686-91
AB  - Interferon signaling is mediated by STATs and interferon regulatory factor (IRF) 
      families of transcription factors. Ten distinct IRFs have been described and most
      are expressed in a variety of cells except for interferon consensus
      sequence-binding protein (ICSBP) and lymphoid-specific IRF/Pip that are thought
      to be exclusively expressed in lymphoid cells. We show here for the first time
      that ICSBP is constitutively and inducibly expressed in the mouse lens. In
      contrast to lymphoid cells with exclusive expression of ICSBP in the nucleus,
      ICSBP is present in both the cytoplasm and nucleus of the lens cell. However,
      ICSBP in the nucleus is of lower apparent molecular weight. We further show that 
      the ICSBP promoter is constitutively bound by lens nuclear factors and that its
      activation requires binding of additional factors including STAT1. Furthermore,
      transcriptional activation of ICSBP gene by interferon gamma is accompanied by
      selective nuclear localization of ICSBP in proliferating epithelial cells but not
      in the nuclei of nondividing cells in the lens fiber compartment. Constitutive
      and inducible expression of ICSBP in the ocular lens and differential regulation 
      of its subcellular localization in the developing lens suggest that ICSBP may
      have nonimmunity related functions and that the commonly held view that it is
      lymphoid-specific be modified.
FAU - Li, W
AU  - Li W
AD  - Laboratory of Immunology, National Institutes of Health, Bethesda, Maryland
      20892, USA.
FAU - Nagineni, C N
AU  - Nagineni CN
FAU - Ge, H
AU  - Ge H
FAU - Efiok, B
AU  - Efiok B
FAU - Chepelinsky, A B
AU  - Chepelinsky AB
FAU - Egwuagu, C E
AU  - Egwuagu CE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (Repressor Proteins)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (Stat1 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (interferon regulatory factor-8)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Consensus Sequence
MH  - DNA-Binding Proteins/metabolism
MH  - Epithelial Cells/metabolism
MH  - Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Interferon Regulatory Factors
MH  - Interferon-gamma/*metabolism
MH  - Lens, Crystalline/*embryology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Repressor Proteins/*biosynthesis/chemistry/genetics
MH  - STAT1 Transcription Factor
MH  - Trans-Activators/metabolism
MH  - *Transcription, Genetic
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1074/jbc.274.14.9686 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 2;274(14):9686-91. doi: 10.1074/jbc.274.14.9686.