PMID- 10092647
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 14
DP  - 1999 Apr 2
TI  - Human pancreatic islets express mRNA species encoding two distinct catalytically 
      active isoforms of group VI phospholipase A2 (iPLA2) that arise from an
      exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 
      gene on chromosome 22q13.1.
PG  - 9607-16
AB  - An 85-kDa Group VI phospholipase A2 enzyme (iPLA2) that does not require Ca2+ for
      catalysis has recently been cloned from three rodent species. A homologous 88-kDa
      enzyme has been cloned from human B-lymphocyte lines that contains a 54-amino
      acid insert not present in the rodent enzymes, but human cells have not
      previously been observed to express catalytically active iPLA2 isoforms other
      than the 88-kDa protein. We have cloned cDNA species that encode two distinct
      iPLA2 isoforms from human pancreatic islet RNA and a human insulinoma cDNA
      library. One isoform is an 85-kDa protein (short isoform of human iPLA2
      (SH-iPLA2)) and the other an 88-kDa protein (long isoform of human iPLA2
      (LH-iPLA2)). Transcripts encoding both isoforms are also observed in human
      promonocytic U937 cells. Recombinant SH-iPLA2 and LH-iPLA2 are both catalytically
      active in the absence of Ca2+ and inhibited by a bromoenol lactone suicide
      substrate, but LH-iPLA2 is activated by ATP, whereas SH-iPLA2 is not. The human
      iPLA2 gene has been found to reside on chromosome 22 in region q13.1 and to
      contain 16 exons represented in the LH-iPLA2 transcript. Exon 8 is not
      represented in the SH-iPLA2 transcript, indicating that it arises by an
      exon-skipping mechanism of alternative splicing. The amino acid sequence encoded 
      by exon 8 of the human iPLA2 gene is proline-rich and shares a consensus motif of
      PX5PX8HHPX12NX4Q with the proline-rich middle linker domains of the Smad proteins
      DAF-3 and Smad4. Expression of mRNA species encoding two active iPLA2 isoforms
      with distinguishable catalytic properties in two different types of human cells
      demonstrated here may have regulatory or functional implications about the roles 
      of products of the iPLA2 gene in cell biologic processes.
FAU - Ma, Z
AU  - Ma Z
AD  - Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, 
      Department of Medicine, Washington University School of Medicine, St. Louis,
      Missouri 63110, USA.
FAU - Wang, X
AU  - Wang X
FAU - Nowatzke, W
AU  - Nowatzke W
FAU - Ramanadham, S
AU  - Ramanadham S
FAU - Turk, J
AU  - Turk J
LA  - eng
SI  - GENBANK/AF102988
SI  - GENBANK/AF102989
SI  - GENBANK/AF116252
SI  - GENBANK/AF116253
SI  - GENBANK/AF116254
SI  - GENBANK/AF116255
SI  - GENBANK/AF116256
SI  - GENBANK/AF116257
SI  - GENBANK/AF116258
SI  - GENBANK/AF116259
SI  - GENBANK/AF116260
SI  - GENBANK/AF116261
SI  - GENBANK/AF116262
SI  - GENBANK/AF116263
SI  - GENBANK/AF116264
SI  - GENBANK/AF116265
SI  - GENBANK/AF116266
SI  - GENBANK/AF116267
SI  - GENBANK/AH009139
GR  - P41 RR000954/RR/NCRR NIH HHS/United States
GR  - R01 DK069455/DK/NIDDK NIH HHS/United States
GR  - R37 DK034388/DK/NIDDK NIH HHS/United States
GR  - R37-DK-34388/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Group VI Phospholipases A2)
RN  - EC 3.1.1.4 (PLA2G6 protein, human)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Catalysis
MH  - Cell Line
MH  - *Chromosomes, Human, Pair 22
MH  - DNA, Complementary/chemistry
MH  - *Exons
MH  - Group VI Phospholipases A2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Islets of Langerhans/*enzymology
MH  - Isoenzymes/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Phospholipases A/*genetics/metabolism
MH  - Phospholipases A2
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Transcription, Genetic
PMC - PMC3715997
MID - NIHMS493627
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1074/jbc.274.14.9607 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 2;274(14):9607-16. doi: 10.1074/jbc.274.14.9607.