PMID- 10092585
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 14
DP  - 1999 Apr 2
TI  - Direct interaction of Alzheimer's disease-related presenilin 1 with armadillo
      protein p0071.
PG  - 9141-8
AB  - Alzheimer's disease-related presenilins are thought to be involved in Notch
      signaling during embryonic development and/or cellular differentiation. Proteins 
      mediating the cellular functions of the presenilins are still unknown. We
      utilized the yeast two-hybrid system to identify an interacting armadillo
      protein, termed p0071, that binds specifically to the hydrophilic loop of
      presenilin 1. In vivo, the presenilins constitutively undergo proteolytic
      processing, forming two stable fragments. Here, we show that the C-terminal
      fragment of presenilin 1 directly binds to p0071. Nine out of 10 armadillo
      repeats in p0071 are essential for mediating this interaction. Since armadillo
      proteins, like beta-catenin and APC, are known to participate in cellular
      signaling, p0071 may function as a mediator of presenilin 1 in signaling events.
FAU - Stahl, B
AU  - Stahl B
AD  - Max Planck Institute for Experimental Medicine, 37075 Gottingen, Germany.
      stahl@mail.mpiem.gwdg.de
FAU - Diehlmann, A
AU  - Diehlmann A
FAU - Sudhof, T C
AU  - Sudhof TC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Ctnnb1 protein, rat)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (PKP4 protein, human)
RN  - 0 (PSEN1 protein, human)
RN  - 0 (PSEN2 protein, human)
RN  - 0 (Plakophilins)
RN  - 0 (Presenilin-1)
RN  - 0 (Presenilin-2)
RN  - 0 (Trans-Activators)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Alzheimer Disease
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/genetics/*metabolism
MH  - Humans
MH  - Membrane Proteins/chemistry/genetics/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Plakophilins
MH  - Presenilin-1
MH  - Presenilin-2
MH  - Protein Binding
MH  - Rats
MH  - Signal Transduction
MH  - *Trans-Activators
MH  - Transfection
MH  - beta Catenin
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1074/jbc.274.14.9141 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 2;274(14):9141-8. doi: 10.1074/jbc.274.14.9141.