PMID- 10092583
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 14
DP  - 1999 Apr 2
TI  - A human protein kinase Bgamma with regulatory phosphorylation sites in the
      activation loop and in the C-terminal hydrophobic domain.
PG  - 9133-6
AB  - We have cloned human protein kinase Bgamma (PKBgamma) and found that it contains 
      two regulatory phosphorylation sites, Thr305 and Ser472, which correspond to
      Thr308 and Ser473 of PKBalpha. Thus it differs significantly from the previously 
      published rat PKBgamma. We have also isolated a similar clone from a mouse cDNA
      library. In human tissues, PKBgamma is widely expressed as two transcripts. A
      mutational analysis of the two regulatory sites of human PKBgamma showed that
      phosphorylation of both sites, occurring in a phosphoinositide 3-kinase-dependent
      manner, is required for full activity. Our results suggest that the two
      phosphorylation sites act in concert to produce full activation of PKBgamma,
      similar to PKBalpha. This contrasts with rat PKBgamma, which is thought to be
      regulated by 3-phosphoinositide-dependent protein kinase 1 alone.
FAU - Brodbeck, D
AU  - Brodbeck D
AD  - Friedrich Miescher-Institut, P. O. Box 2543, 4002 Basel, Switzerland.
FAU - Cron, P
AU  - Cron P
FAU - Hemmings, B A
AU  - Hemmings BA
LA  - eng
SI  - GENBANK/AF124141
SI  - GENBANK/AF124142
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Oncogene Proteins)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.1 (AKT3 protein, human)
RN  - EC 2.7.11.1 (Akt3 protein, mouse)
RN  - EC 2.7.11.1 (Akt3 protein, rat)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Catalytic Domain
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA Mutational Analysis
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oncogene Proteins/chemistry/*metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/chemistry/*metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - Rats
MH  - Sequence Alignment
MH  - Serine/metabolism
MH  - Structure-Activity Relationship
MH  - Threonine/metabolism
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - 10.1074/jbc.274.14.9133 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Apr 2;274(14):9133-6. doi: 10.1074/jbc.274.14.9133.