PMID- 10092534
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 257
IP  - 1
DP  - 1999 Apr 2
TI  - Molecular cloning of a novel 130-kDa cytoplasmic protein, Ankhzn, containing
      Ankyrin repeats hooked to a zinc finger motif.
PG  - 206-13
AB  - A novel gene was trapped in mouse embryonic stem cells with a promoterless gene
      trap vector. Fused transcripts were isolated from the embryos by rapid
      amplification of cDNA ends, which were used for full-length cDNA cloning. The
      protein predicted from the cDNA consisting of 7143 nucleotides comprises 1184
      amino acids, which was confirmed by in vitro transcription/translation assaying. 
      An antibody against the synthesized peptide reacted with an approximate 130-kDa
      protein on SDS-PAGE. A search of available databases revealed that this protein
      is a novel protein composed of 17 ankyrin repeats hooked to a zinc finger motif, 
      which we named Ankhzn. Ankhzn was observed on the endosomal membrane on
      immunoelectron microscopic analysis. Ankhzn belongs to a new subgroup of double
      zinc finger proteins which may be involved in vesicle or protein transport.
      Ankhzn mRNA and its protein were expressed ubiquitously from embryonic day 10.5
      to adulthood.
CI  - Copyright 1999 Academic Press.
FAU - Ito, K
AU  - Ito K
AD  - Research Laboratory for Molecular Genetics, Niigata University, Niigata,
      951-8510, Japan.
FAU - Ishii, N
AU  - Ishii N
FAU - Miyashita, A
AU  - Miyashita A
FAU - Tominaga, K
AU  - Tominaga K
FAU - Kuriyama, H
AU  - Kuriyama H
FAU - Maruyama, H
AU  - Maruyama H
FAU - Shirai, M
AU  - Shirai M
FAU - Naito, M
AU  - Naito M
FAU - Arakawa, M
AU  - Arakawa M
FAU - Kuwano, R
AU  - Kuwano R
LA  - eng
SI  - GENBANK/AB011370
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Ankfy1 protein, mouse)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Ankyrin Repeat/*genetics
MH  - Base Sequence
MH  - Brain/embryology/growth & development/metabolism
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Cell Line
MH  - Cell Membrane/metabolism/ultrastructure
MH  - Cloning, Molecular
MH  - Cytoplasm/*metabolism/ultrastructure
MH  - DNA, Complementary/genetics
MH  - Endosomes/metabolism/ultrastructure
MH  - Gene Expression Regulation, Developmental
MH  - Kidney/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Open Reading Frames/genetics
MH  - RNA, Messenger/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/chemistry/genetics
MH  - Stem Cells/metabolism
MH  - Zinc Fingers/*genetics
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - S0006-291X(99)90430-8 [pii]
AID - 10.1006/bbrc.1999.0430 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 2;257(1):206-13. doi:
      10.1006/bbrc.1999.0430.