PMID- 10092522
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 257
IP  - 1
DP  - 1999 Apr 2
TI  - The proto-oncogene p120(Cbl) is a downstream substrate of the Hck
      protein-tyrosine kinase.
PG  - 129-38
AB  - Hematopoietic cell kinase (Hck) is a member of the Src-family of protein tyrosine
      kinases. We have found that upon enzymatic activation of Hck by the heavy metal
      mercuric chloride, there was a rapid increase in the levels of tyrosine
      phosphorylation of several proteins including the proto-oncogene p120(Cbl).
      Fibroblasts that are transformed with an activated allele of Hck exhibit
      constitutive Cbl phosphorylation. Upon Fcgamma receptor activation, a more
      physiologically relevant extracellular signal, Cbl is tyrosine phosphorylated and
      the Src-family selective inhibitor, PP1, can prevent this phosphorylation on Cbl.
      Hck phosphorylates Cbl in vitro and the interaction between Cbl and Hck is
      direct, requiring Hck's unique, SH3 and SH2 domains for optimal binding. Using a 
      novel estrogen-regulated chimera of Hck we have shown a hormone-dependent
      association between Hck and Cbl in murine fibroblasts. This work suggests that
      Cbl serves as a key mediator of Hck induced signalling in hematopoietic cells.
CI  - Copyright 1999 Academic Press.
FAU - Howlett, C J
AU  - Howlett CJ
AD  - Department of Oncology, University of Calgary, Calgary, Alberta, Canada.
FAU - Bisson, S A
AU  - Bisson SA
FAU - Resek, M E
AU  - Resek ME
FAU - Tigley, A W
AU  - Tigley AW
FAU - Robbins, S M
AU  - Robbins SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (4-amino-5-(4-methylphenyl)-7-(tert-butyl)pyrazolo(3,4-d)pyrimidine)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyrimidines)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 4TI98Z838E (Estradiol)
RN  - 53GH7MZT1R (Mercuric Chloride)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-cbl)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (HCK protein, human)
RN  - EC 2.7.10.2 (Hck protein, mouse)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-hck)
RN  - EC 6.3.2.- (CBL protein, human)
RN  - EC 6.3.2.- (Cbl protein, mouse)
SB  - IM
MH  - 3T3 Cells
MH  - Alleles
MH  - Animals
MH  - Cell Line, Transformed
MH  - Enzyme Activation/drug effects
MH  - Estradiol/pharmacology
MH  - Humans
MH  - Mercuric Chloride/pharmacology
MH  - Mice
MH  - Phosphorylation/drug effects
MH  - Phosphotyrosine/metabolism
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein-Tyrosine Kinases/genetics/*metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-cbl
MH  - Proto-Oncogene Proteins c-hck
MH  - Pyrazoles/pharmacology
MH  - Pyrimidines/pharmacology
MH  - Receptor Aggregation
MH  - Receptors, IgG/antagonists & inhibitors/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - *Ubiquitin-Protein Ligases
MH  - src Homology Domains/physiology
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - S0006-291X(99)90427-8 [pii]
AID - 10.1006/bbrc.1999.0427 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 2;257(1):129-38. doi:
      10.1006/bbrc.1999.0427.