PMID- 10092517
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 257
IP  - 1
DP  - 1999 Apr 2
TI  - Identification of NEDD8-conjugation site in human cullin-2.
PG  - 100-5
AB  - NEDD8 is a novel ubiquitin-like protein that has been shown to conjugate to
      nuclear proteins in a manner analogous to ubiquitination and sentrinization.
      Recently, human cullin-4A was reported to be conjugated by a single molecule of
      NEDD8. Here, we show that human cullin-2 is also conjugated by a single molecule 
      of the NEDD8. The C-terminal 171-amino-acid residues in human cullin-2 are
      sufficient for NEDD8-conjugation. In addition, the equivalent C-terminal
      fragments of other cullins have been shown to be conjugated by NEDD8. Mapping of 
      the NEDD8-conjugation site revealed that Lys-689 in human cullin-2 is conjugated 
      by NEDD8. Interestingly, the Lys residue at position 689 in cullin-2 is conserved
      in all cullin family members, including human cullin-1, -2, -3, -4A, -4B, and -5 
      and yeast cullin (Cdc53), suggesting the possibility that other cullin family
      members are conjugated by NEDD8/Rub1 at a Lys residue of equivalent position.
CI  - Copyright 1999 Academic Press.
FAU - Wada, H
AU  - Wada H
AD  - Department of Internal Medicine, Institute of Molecular Medicine for the
      Prevention of Human Diseases, Houston, Texas 77030, USA.
FAU - Yeh, E T
AU  - Yeh ET
FAU - Kamitani, T
AU  - Kamitani T
LA  - eng
SI  - GENBANK/AF126404
GR  - HL-45851/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CUL2 protein, human)
RN  - 0 (CUL4A protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cdc53 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cullin Proteins)
RN  - 0 (NEDD8 Protein)
RN  - 0 (NEDD8 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Ubiquitins)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - Binding Sites
MH  - Blotting, Western
MH  - COS Cells
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cell Cycle Proteins/chemistry/genetics/*metabolism
MH  - Conserved Sequence
MH  - *Cullin Proteins
MH  - Humans
MH  - Lysine/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - NEDD8 Protein
MH  - Neoplasm Proteins/chemistry/genetics
MH  - Peptide Fragments/chemistry/genetics/isolation & purification/metabolism
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/biosynthesis/chemistry/isolation &
      purification/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Deletion
MH  - Transfection
MH  - Ubiquitins/*metabolism
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - S0006-291X(99)90339-X [pii]
AID - 10.1006/bbrc.1999.0339 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 2;257(1):100-5. doi: 10.1006/bbrc.1999.0339.