PMID- 10092508
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 257
IP  - 1
DP  - 1999 Apr 2
TI  - Cloning and expression of a novel lysophospholipase which structurally resembles 
      lecithin cholesterol acyltransferase.
PG  - 50-6
AB  - Lecithin cholesterol acyltransferase (LCAT) is the key enzyme in the
      esterification of plasma cholesterol and in the reverse cholesterol transport on 
      high-density lipoprotein (HDL). We have found a novel LCAT-related gene among
      differentially expressed cDNA fragments between two types of foam cells derived
      from THP-1 cells, which are different in cholesterol efflux ability, using a
      subtractive PCR technique. The deduced 412-amino-acid sequence has 49% amino acid
      sequence similarity with human LCAT. In contrast to the liver-specific expression
      of LCAT, mRNA expression of the gene was observed mainly in peripheral tissues
      including kidney, placenta, pancreas, testis, spleen, heart, and skeletal muscle.
      The protein exists in human plasma and is probably associated with HDL. Moreover,
      we discovered that the recombinant protein hydrolyzed lysophosphatidylcholine
      (lysoPC), a proatherogenic lipid, to glycerophosphorylcholine and a free fatty
      acid. We have therefore named this novel enzyme LCAT-like lysophospholipase
      (LLPL), through which a new catabolic pathway for lysoPC on lipoproteins could be
      elucidated.
CI  - Copyright 1999 Academic Press.
FAU - Taniyama, Y
AU  - Taniyama Y
AD  - Pharmaceutical Discovery Research Division, Takeda Chemical Industries Ltd., 10
      Wadai, Tsukuba, Ibaraki, 300-4293, Japan. Taniyama_Yoshio@takeda.co.jp
FAU - Shibata, S
AU  - Shibata S
FAU - Kita, S
AU  - Kita S
FAU - Horikoshi, K
AU  - Horikoshi K
FAU - Fuse, H
AU  - Fuse H
FAU - Shirafuji, H
AU  - Shirafuji H
FAU - Sumino, Y
AU  - Sumino Y
FAU - Fujino, M
AU  - Fujino M
LA  - eng
SI  - GENBANK/AB017494
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Butyrates)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 12UHW9R67N (Isoflurophate)
RN  - 2635-84-9 (4-nitrophenyl butyrate)
RN  - EC 2.3.1.43 (Phosphatidylcholine-Sterol O-Acyltransferase)
RN  - EC 3.1.1.5 (Lysophospholipase)
RN  - EDS2L3ODLV (1,2-oleoylphosphatidylcholine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Baculoviridae/genetics
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Butyrates/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Foam Cells/enzymology/metabolism
MH  - Humans
MH  - Isoflurophate/pharmacology
MH  - Kinetics
MH  - Lysophosphatidylcholines/metabolism
MH  - Lysophospholipase/blood/*chemistry/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phosphatidylcholine-Sterol O-Acyltransferase/*chemistry/genetics
MH  - Phosphatidylcholines/metabolism
MH  - RNA, Messenger/analysis
MH  - Recombinant Fusion Proteins/biosynthesis/chemistry/isolation &
      purification/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/27 00:00
MHDA- 1999/03/27 00:01
CRDT- 1999/03/27 00:00
PHST- 1999/03/27 00:00 [pubmed]
PHST- 1999/03/27 00:01 [medline]
PHST- 1999/03/27 00:00 [entrez]
AID - S0006-291X(99)90411-4 [pii]
AID - 10.1006/bbrc.1999.0411 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Apr 2;257(1):50-6. doi: 10.1006/bbrc.1999.0411.