PMID- 10092231
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 283
IP  - 5410
DP  - 1999 Mar 26
TI  - Inhibition of myosin light chain kinase by p21-activated kinase.
PG  - 2083-5
AB  - p21-activated kinases (PAKs) are implicated in the cytoskeletal changes induced
      by the Rho family of guanosine triphosphatases. Cytoskeletal dynamics are
      primarily modulated by interactions of actin and myosin II that are regulated by 
      myosin light chain kinase (MLCK)-mediated phosphorylation of the regulatory
      myosin light chain (MLC). p21-activated kinase 1 (PAK1) phosphorylates MLCK,
      resulting in decreased MLCK activity. MLCK activity and MLC phosphorylation were 
      decreased, and cell spreading was inhibited in baby hamster kidney-21 and HeLa
      cells expressing constitutively active PAK1. These data indicate that MLCK is a
      target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing
      MLCK activity and MLC phosphorylation.
FAU - Sanders, L C
AU  - Sanders LC
AD  - Departments of Immunology and Cell Biology, The Scripps Research Institute, La
      Jolla, CA 92037, USA.
FAU - Matsumura, F
AU  - Matsumura F
FAU - Bokoch, G M
AU  - Bokoch GM
FAU - de Lanerolle, P
AU  - de Lanerolle P
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Myosin Light Chains)
RN  - 17885-08-4 (Phosphoserine)
RN  - 19SQ93LM6H (diacetylmonoxime)
RN  - EC 2.7.11.1 (PAK1 protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 2.7.11.18 (Myosin-Light-Chain Kinase)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.4.1 (Myosins)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - K324J5K4HM (Diacetyl)
SB  - IM
CIN - Science. 1999 Mar 26;283(5410):2028-9. PMID: 10206910
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Cycle Proteins/metabolism
MH  - Cell Line
MH  - Cell Movement
MH  - Cell Size
MH  - Cricetinae
MH  - Cytoskeleton/*physiology
MH  - Diacetyl/analogs & derivatives/pharmacology
MH  - GTP Phosphohydrolases/metabolism
MH  - GTP-Binding Proteins/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Myosin Light Chains/*metabolism
MH  - Myosin-Light-Chain Kinase/*antagonists & inhibitors/metabolism
MH  - Myosins/physiology
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Signal Transduction
MH  - cdc42 GTP-Binding Protein
MH  - p21-Activated Kinases
MH  - rac GTP-Binding Proteins
MH  - rho-Associated Kinases
EDAT- 1999/03/26 00:00
MHDA- 1999/03/26 00:01
CRDT- 1999/03/26 00:00
PHST- 1999/03/26 00:00 [pubmed]
PHST- 1999/03/26 00:01 [medline]
PHST- 1999/03/26 00:00 [entrez]
AID - 10.1126/science.283.5410.2083 [doi]
PST - ppublish
SO  - Science. 1999 Mar 26;283(5410):2083-5. doi: 10.1126/science.283.5410.2083.