PMID- 10092119
OWN - NLM
STAT- MEDLINE
DCOM- 19990511
LR  - 20211203
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 52
IP  - 4
DP  - 1999 Apr
TI  - Tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes
      multiple testis transcripts.
PG  - 392-405
AB  - Hypothesizing that genes important in meiotic processes in mammals might have
      evolutionarily conserved counterparts in lower organisms, we used the yeast IME2 
      meiotic gene (serine threonine kinase) as a probe for screening a mouse testis
      cDNA library. This screening resulted in identification of a novel putative
      serine threonine kinase. Although it did not exhibit significant homology to
      IME2, it did show significant sequence homology to the Tousled kinase in
      Arabidopsis. Tousled is associated with various differentiative processes
      including differentiation of the reproductive organs. The new murine gene was
      designated accordingly Tlk (Tousled like kinase). Tousled like kinase sequences
      have been reported to occur in C. elegans and in the human. Positive
      hybridization signals obtained in zooblot analysis suggest evolutionary
      conservation of Tlk throughout the phylogenetic ladder. Four distinct Tlk
      transcripts were detected in mouse testis, at least one of which is
      testis-specific. Northern and in situ hybridization analyses revealed that in
      normal testis, Tlk is expressed predominantly in pachytene spermatocytes and in
      round spermatids. Transcripts differ from one another in their 3' untranslated
      region, resulting from use of different polyadenylation sites, and in the length 
      of their 5' region. Within the coding region, three of the putative peptides
      share the kinase and C-terminal domains but differ in their N-terminal domain,
      suggesting that the latter may be involved in the regulation of Tlk's function.
      We conclude that although Tlk might have an essential role in all tissues, these 
      kinases are likely to take part in the complex array of phosphorylations involved
      in regulating spermatogenesis.
FAU - Shalom, S
AU  - Shalom S
AD  - Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
FAU - Don, J
AU  - Don J
LA  - eng
SI  - GENBANK/AF045252
SI  - GENBANK/AF045253
SI  - GENBANK/AF045254
SI  - GENBANK/U56909
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (DNA, Complementary)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (TLK-1 protein, C elegans)
RN  - EC 2.7.1.- (Tlk1 protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TLK1 protein, human)
SB  - IM
MH  - Aging/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Caenorhabditis elegans Proteins
MH  - Conserved Sequence
MH  - DNA, Complementary
MH  - *Evolution, Molecular
MH  - *Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Protein Kinases/chemistry/genetics
MH  - Protein Serine-Threonine Kinases/biosynthesis/chemistry/*genetics
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Testis/*enzymology/growth & development
MH  - *Transcription, Genetic
EDAT- 1999/03/26 00:00
MHDA- 2000/06/20 00:00
CRDT- 1999/03/26 00:00
PHST- 1999/03/26 00:00 [pubmed]
PHST- 2000/06/20 00:00 [medline]
PHST- 1999/03/26 00:00 [entrez]
AID - 10.1002/(SICI)1098-2795(199904)52:4<392::AID-MRD8>3.0.CO;2-Y [pii]
AID - 10.1002/(SICI)1098-2795(199904)52:4<392::AID-MRD8>3.0.CO;2-Y [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1999 Apr;52(4):392-405. doi:
      10.1002/(SICI)1098-2795(199904)52:4<392::AID-MRD8>3.0.CO;2-Y.