PMID- 10090575
OWN - NLM
STAT- MEDLINE
DCOM- 19990430
LR  - 20191103
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 111
IP  - 2
DP  - 1999 Feb
TI  - Regional expression of sulfatides in rat kidney: immunohistochemical staining by 
      use of monospecific polyclonal antibodies.
PG  - 143-51
AB  - Sulfatides are quantitatively prominent glycosphingolipids of rat kidney. In
      order to gain insight into their possible physiological significance in this
      organ, and their possible role in Heymann's nephritis, the main sulfatide
      components were localized immunohistochemically. The antibodies used recognized
      the sulfatides Sgal1, Stri1, Stri2, and Stet2a. Stri1 epitopes were expressed in 
      the brush border of proximal tubuli, whereas Stri2-specific immuno staining was
      observed in cortical and medullar collecting ducts. Both Stri1 and Stri2 were
      also expressed by interstitial cells of the inner medulla. Stet2a was visualized 
      in epithelia of the distal tubules of Henle's loop and the juxtaglomerular
      apparatus, including the macula densa. The mAb Sulph-I, that recognizes the
      SO3(-)-3Galbeta<epitope of Sgal1, seminolipid, and Slac1, prominently stained the
      cortical and medullar collecting ducts. No sulfatide was immuno-stained in the
      glomerular region. For this reason, the possibility of a direct involvement of
      anti-Stri1 and Stri2 antibodies in the etiology of Heymann's nephritis appears to
      be less likely. A strict correlation between the local sulfatide content, as
      analyzed by chemical estimation, and the sulfatide expression, as observed by
      immunohistochemical methods, may not be possible.
FAU - Trick, D
AU  - Trick D
AD  - Physiologisch-Chemisches Institut, Philipps-Universitat, Marburg, Germany.
FAU - Decker, J
AU  - Decker J
FAU - Groene, H J
AU  - Groene HJ
FAU - Schulze, M
AU  - Schulze M
FAU - Wiegandt, H
AU  - Wiegandt H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Glycolipids)
RN  - 0 (Immune Sera)
RN  - 0 (Sulfoglycosphingolipids)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity
MH  - Antigen-Antibody Reactions
MH  - Epitopes/analysis/chemistry/immunology
MH  - Glycolipids/immunology
MH  - Immune Sera
MH  - Immunohistochemistry
MH  - Kidney/*chemistry
MH  - Rabbits
MH  - Rats
MH  - Sulfoglycosphingolipids/*analysis/chemistry/immunology
EDAT- 1999/03/25 00:00
MHDA- 1999/03/25 00:01
CRDT- 1999/03/25 00:00
PHST- 1999/03/25 00:00 [pubmed]
PHST- 1999/03/25 00:01 [medline]
PHST- 1999/03/25 00:00 [entrez]
AID - 10.1007/s004180050344 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 1999 Feb;111(2):143-51. doi: 10.1007/s004180050344.