PMID- 10090333
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20131121
IS  - 0001-6772 (Print)
IS  - 0001-6772 (Linking)
VI  - 165
IP  - 2
DP  - 1999 Feb
TI  - Carbonic anhydrase IV activity is localized on the exterior surface of human
      erythrocytes.
PG  - 211-8
AB  - Carbonic anhydrase (CA) cytoplasmic isozymes CA I and CA II were found in human
      erythrocyte membrane ghosts, when prepared at pH 5.4 and pH 7.4, but not in
      ghosts prepared at pH 8.2. These findings could indicate that previously reported
      CA activity of ghosts was owing to contamination by CA I and CA II during the
      preparation of the ghosts. However, using a sensitive micro-assay, CA activity
      was also found in ghosts prepared at pH 8.2. This activity constitutes 0.2% of
      the erythrocytes' total CA-activity, and originates from a membrane-associated
      isoform of CA, located at the exterior membrane surface. It has immunochemical
      and kinetic properties like those of the membrane-bound CA IV, previously
      isolated from kidney, lung and small blood vessels. Its function is possibly to
      interact with the red cell membrane anionic transport protein, band 3, for the
      bicarbonate/chloride exchange.
FAU - Wistrand, P J
AU  - Wistrand PJ
AD  - Department of Medical Pharmacology, University of Uppsala, Biomedical Center,
      Sweden.
FAU - Carter, N D
AU  - Carter ND
FAU - Conroy, C W
AU  - Conroy CW
FAU - Mahieu, I
AU  - Mahieu I
LA  - eng
GR  - EY-02227/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Acta Physiol Scand
JT  - Acta physiologica Scandinavica
JID - 0370362
RN  - 0 (Carbonic Anhydrase Inhibitors)
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - Z52H4811WX (Ethoxzolamide)
SB  - IM
MH  - Absorption
MH  - Animals
MH  - Blotting, Western
MH  - Carbon Dioxide/metabolism
MH  - Carbonic Anhydrase Inhibitors/pharmacology
MH  - Carbonic Anhydrases/drug effects/*metabolism
MH  - Cattle
MH  - Cell Membrane Permeability/drug effects
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Erythrocyte Membrane/drug effects/*enzymology
MH  - Ethoxzolamide/pharmacology
MH  - Humans
MH  - Immunodiffusion
EDAT- 1999/03/25 00:00
MHDA- 1999/03/25 00:01
CRDT- 1999/03/25 00:00
PHST- 1999/03/25 00:00 [pubmed]
PHST- 1999/03/25 00:01 [medline]
PHST- 1999/03/25 00:00 [entrez]
AID - 10.1046/j.1365-201x.1999.00478.x [doi]
PST - ppublish
SO  - Acta Physiol Scand. 1999 Feb;165(2):211-8. doi: 10.1046/j.1365-201x.1999.00478.x.